EN
Hepa 1-6 Mouse Liver Cancer Cell Line with RFP
Introduction
The Hepa 1-6 mouse liver cancer cell line is a derivative of the BW7756 tumor originally established in C57/L mice. The cell line has been tested and confirmed to be ectromelia virus (ECTV) negative. OriCell™ Hepa 1-6 Mouse Liver Cancer Cell Line is widely used in hepatocellular carcinoma research, in vivo tumor model establishment, and pharmacological studies.
OriCell™ Hepa 1-6 Mouse Liver Cancer Cell Line with RFP is generated by transducing Hepa 1-6 cells with a recombinant lentiviral vector carrying the RFP gene, resulting in stable expression of red fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ Hepa 1-6 Mouse Liver Cancer Cell Line with RFP |
| Alternative Name | Hepa 1-6 with RFP |
| Catalog Number | M1-0204 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Liver |
| Cell Characteristics | Adherent Growth; Epithelial-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 48 ~ 72 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
