NIH/3T3 Mouse Embryonic Fibroblast Cell Line

Introduction

The NIH/3T3 Mouse Embryonic Fibroblast Cell Line, established from NIH/Swiss mouse embryo cultures, is a continuous cell line. NIH/3T3 cells are highly sensitive to foci formation by sarcoma viruses and the replication of leukemia viruses, making them valuable for DNA transformation and transfection studies. This cell line is ectromelia virus (mousepox) negative.

Due to its high transfection efficiency, OriCell™ NIH/3T3 Mouse Embryonic Fibroblast Cell Line is commonly used in laboratory research involving transfection and gene expression studies.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing.
• Verified by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.