EN
GL261 Mouse Glioma Cell Line with GFP
Introduction
The GL261 mouse glioma cell line was established from a glioblastoma in C57BL/6 mice and is known for harboring TP53 and KRAS mutations. It is commonly used as a model for glioblastoma research. These cells have been reported to contract in the presence of 1 μM angiotensin II. Tumors are induced via intracranial injection of 3-methylcholanthrene and maintained through serial transplantation in syngeneic mice.
OriCell™ GL261 Mouse Glioma Cell Line is widely used in studies of glioblastoma pathogenesis, as well as in preclinical screening and evaluation of immunotherapeutic and radiotherapeutic agents. It is also used to investigate interactions between tumors and the gut microbiota.
OriCell™ GL261 Mouse Glioma Cell Line with GFP is generated by transducing the parental GL261 cell line with a recombinant lentivirus vector carrying the GFP gene, resulting in stable expression of green fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ GL261 Mouse Glioma Cell Line with GFP |
| Alternative Name | GL261 with GFP |
| Catalog Number | M8-0303 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Central Nervous System Glia |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 48 ~ 72 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If GFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
