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RAW 264.7 Mouse Monocyte Macrophage Leukemia Cell Line
Introduction
The RAW 264.7 mouse monocyte macrophage leukemia cell line was originally isolated from tumor tissues of male BALB/c mice induced by the Abelson murine leukemia virus (A-MuLV). This cell line is characterized by its ease of culture and passage, sensitivity to RNA interference (RNAi), and high DNA transfection efficiency, making it a widely used host for transfection studies and a model for murine norovirus (MNV) replication.
The cells are negative for the surface markers sIg⁻, Ia⁻, and Thy-1.2. It has been reported that the established cell line no longer secretes or contains detectable viral particles. Furthermore, the cell line has tested negative in XC plaque assays and for the presence of the ectromelia virus (mousepox).
RAW 264.7 cells are extensively utilized in research areas such as oxidative stress, inflammation, and antibacterial activity. In immunology, they serve as a critical model for investigating macrophage immune response mechanisms, including the production and function of cytokines. In inflammation-related studies, this cell line is used to simulate in vivo inflammatory responses and to screen for potential anti-inflammatory drugs.
All batches of this cell line undergo rigorous quality validation, including cell identity authentication, morphological evaluation, viability testing, and mycoplasma screening. This ensures high experimental reproducibility and data reliability.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ RAW 264.7 Mouse Monocyte Macrophage Leukemia Cell Line |
| Alternative Name | RAW 264.7 |
| Catalog Number | M3-0101 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Ascites |
| Cell Characteristics | Adherent Growth; Macrophage-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 2 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Avoid using trypsin. It is recommended to use culture dishes and high-quality serum. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
To view RAW 264.7 cell doubling and growth status, click here.
