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BV-2 Mouse Microglial Cell Line with Luciferase & GFP
Introduction
The BV-2 mouse microglial cell line was established by E. Blasi in 1990. BV-2 cells were immortalized through retroviral transduction of v-raf/v-myc into mouse microglial cells. These cells retain multiple morphological, phenotypic, and functional characteristics of primary microglia. Immunocytochemical analysis shows that BV-2 cells are positive for MAC1 and MAC2, but negative for MAC3, glial fibrillary acidic protein (GFAP), and galactocerebroside (GalC).
OriCell™ BV-2 Mouse Microglial Cell Line is commonly used in neuroinflammation research, including studies on the inhibitory effects of MIF enzyme activity inhibitors and compounds such as sinomenine. OriCell™ BV-2 Mouse Microglial Cell Line with Luciferase & GFP is generated by transducing BV-2 cells with a recombinant lentiviral vector carrying the luciferase and GFP genes, resulting in stable expression of luciferase and green fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ BV-2 Mouse Microglial Cell Line with Luciferase & GFP |
| Alternative Name | BV-2 with Luciferase & GFP |
| Catalog Number | M8-0705 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Brain |
| Cell Characteristics | Semi-adherent Growth; Epithelial-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | MEM + 10% FBS (with NEAA) |
| Doubling Time | 24 ~ 48 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If GFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
