MH-S Mouse Alveolar Macrophage Cell Line
Introduction
The MH-S mouse alveolar macrophage cell line was derived from the lungs of a 7-week-old male BALB/c mouse and established via SV40-mediated transformation of primary alveolar macrophages. This cell line expresses antigens such as CD11b, I-A, and T, and can produce IL-1 upon stimulation with lipopolysaccharide (LPS).
OriCell™ MH-S Mouse Alveolar Macrophage Cell Line is commonly used in immunological research, for example, to investigate the anti-inflammatory mechanisms of Relinqing Granules in LPS-induced murine alveolar macrophages via the TLR4/MyD88/NF-κB signaling pathway.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ MH-S Mouse Alveolar Macrophage Cell Line |
| Alternative Name | MH-S |
| Catalog Number | M0-0401 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Lung |
| Cell Characteristics | Semi-adherent Growth; Macrophage-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | RPMI-1640 + 10% FBS |
| Doubling Time | 24 ~ 48 h |
| Biosafety Level | 2 |
| Storage at | Liquid Nitrogen (-196℃) |
| Precautions | —— |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.