EN
Technical Bulletin
| Exosome Isolation and Characterization Methods: A Practical Guide |
| Jul 01, 2026 |
| Explore commonly used exosome isolation and characterization methods, including ultracentrifugation, density gradient centrifugation, ultrafiltration, immunoaffinity isolation, size-exclusion chromatography, TEM, NTA, and Western blotting. This practical guide also introduces OriCell exosome research services, including stem cell products, exosome-depleted media and serum, and customized exosome isolation and characterization support. Read More > |
| How to Generate Embryoid Bodies from Embryonic Stem Cells: A Standardized ESC Differentiation Protocol |
| Jun 30, 2026 |
| Generate uniform, high-quality embryoid bodies from embryonic stem cells with this standardized ESC differentiation protocol. This article covers suspension culture preparation, adherent induction, molecular mechanisms of embryoid body differentiation, and key quality control tips, with recommended OriCell mouse ESCs and embryoid body formation medium. Read More > |
| Why Your MSC Osteogenic Induction Fails: Key Steps and Troubleshooting for Mesenchymal Stem Cell Osteogenesis |
| Jun 26, 2026 |
| Master MSC osteogenic induction with a complete step-by-step protocol covering cell coating, seeding, induction initiation, and Alizarin Red staining. Includes troubleshooting for common issues like poor staining, nodule detachment, and induction failure, with recommended OriCell osteogenic differentiation kits. Read More > |
| Cell Culture Fungal Contamination: What It Is, Where It Comes From, and How to Fix It |
| Jun 25, 2026 |
| Effectively detect and prevent fungal contamination in cell culture with standardized identification methods and antifungal solutions. Includes visual and microscopic detection criteria for mold and yeast, complete decontamination protocols, and recommended OriCell antibiotic-antimycotic products for routine contamination prevention. Read More > |
| Do You Really Need Antibiotics in Cell Culture? Risks, Best Practices, and Alternatives |
| Apr 22, 2026 |
| In cell culture, the use of antibiotics remains a debated topic. Are they truly necessary? Why does contamination sometimes persist even with antibiotics present? Read More > |
| Beyond the Textbook: Navigating the Stem Cell Potency Spectrum for Research Design |
| Dec 24, 2025 |
| Practical guide to totipotent, pluripotent (ESCs, iPSCs), and multipotent (MSCs) with markers, applications, and model selection strategies for experiments. Read More > |
| Download the MSCs Culture and Trilineage Differentiation Guide (PDF): SOPs, Protocols, and Troubleshooting |
| Dec 16, 2025 |
| Practical blog introducing the downloadable MSC culture and tri‑lineage differentiation guide, covering confluence control, passage range (P3–P6), osteogenic/adipogenic/chondrogenic tips, staining, troubleshooting, and related Cyagen OriCell™ products. Read More > |
| DMSO, the Classic and Practical Cryoprotectant — Chances Are You've Used It |
| Dec 12, 2025 |
| Practical overview of dimethyl sulfoxide (DMSO) in cell culture and cryopreservation, covering physical/chemical properties, cryoprotective mechanisms, toxicity considerations, 3Rs (remove/reduce/replace), thawing practice, slow‑freeze/rapid‑thaw, and key references. Read More > |
| How to Identify Mesenchymal Stem Cells (MSCs) |
| Dec 04, 2025 |
| Practical MSC identification guide covering morphology, flow cytometry markers (CD73/CD90/CD105; CD45/CD34/CD14/CD11b/CD79α/CD19/HLA‑DR), and osteogenic/adipogenic/chondrogenic differentiation, with related OriCell products. Read More > |
| Trilineage Differentiation and Identification of Mesenchymal Stem Cells (MSCs) |
| Nov 27, 2025 |
| Overview of MSC osteogenic, adipogenic, and chondrogenic differentiation; in vitro identification by ALP, Oil Red O, Alizarin Red S, Collagen II; common markers and assays. Read More > |
