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RAW 264.7 Mouse Monocyte Macrophage Leukemia Cell Line with RFP
Introduction
The RAW 264.7 mouse monocyte macrophage leukemia cell line was originally isolated from tumor tissues of male BALB/c mice induced by the Abelson murine leukemia virus (A-MuLV). This cell line is characterized by its ease of culture and passage, sensitivity to RNA interference (RNAi), and high DNA transfection efficiency, making it a widely used host for transfection studies and a model for murine norovirus (MNV) replication.
The cells are negative for the surface markers sIg⁻, Ia⁻, and Thy-1.2. It has been reported that the established cell line no longer secretes or contains detectable viral particles. Furthermore, the cell line has tested negative in XC plaque assays and for the presence of the ectromelia virus (mousepox).
OriCell™ RAW 264.7 Mouse Monocyte Macrophage Leukemia Cell Line with RFP is a stable cell line constitutively expressing red fluorescent protein, generated by transducing the parental RAW 264.7 line with a lentiviral vector harboring the RFP gene. This modified line serves as an optimized tool for a wide range of in vivo and in vitro applications requiring precise cellular tracking and visualization.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ RAW 264.7 Mouse Monocyte Macrophage Leukemia Cell Line with RFP |
| Alternative Name | RAW 264.7 with RFP |
| Catalog Number | M3-0104 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Ascites |
| Cell Characteristics | Adherent Growth; Macrophage-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 2 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Avoid using trypsin. It is recommended to use culture dishes and high-quality serum. |
| Tips | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
