EN
Pan02 Mouse Pancreatic Cancer Cell Line with RFP
Introduction
The Pan02 mouse pancreatic cancer cell line (also known as Panc02) is derived from mouse pancreatic tissue and serves as a classic cellular model for pancreatic cancer research. This cell line can be used to establish syngeneic tumor models in immunocompetent C57BL/6 mice, enabling studies of interactions between tumors and the host immune system.
OriCell™ Pan02 Mouse Pancreatic Cancer Cell Line is widely used in pancreatic cancer research, including 3D tumor model development, tumor microenvironment studies, immunotherapy evaluation, and anticancer drug screening and efficacy assessment.
OriCell™ Pan02 Mouse Pancreatic Cancer Cell Line with RFP is generated by transducing Pan02 cells with a recombinant lentiviral vector carrying the RFP gene, resulting in stable expression of red fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ Pan02 Mouse Pancreatic Cancer Cell Line with RFP |
| Alternative Name | Pan02 with RFP |
| Catalog Number | M2-0504 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Pancreas |
| Cell Characteristics | Adherent Growth; Epithelial-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 5% FBS |
| Doubling Time | 48 ~ 72 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
