EN
DC2.4 Mouse Bone Marrow-Derived Dendritic Cell Line
Introduction
The DC2.4 mouse bone marrow-derived dendritic cell line was established by Kenneth L. Rock’s laboratory through retrovirus-mediated oncogene transfection and immortalization of bone marrow-derived dendritic cells from C57BL/6 mice (H-2Kᵇ). The cells exhibit typical dendritic cell characteristics, growing as suspension or semi-adherent cells with irregular round or spindle-shaped morphology. They express dendritic cell-specific markers and possess the capacity for phagocytosis as well as the presentation of exogenous antigens on MHC class I and class II molecules.
OriCell™ DC2.4 Mouse Bone Marrow-Derived Dendritic Cell Line is widely used in studies of cancer immunotherapy, drug and adjuvant screening, antigen presentation, and T cell activation. It has been applied in research such as investigating the effects of Lycium barbarum polysaccharides on DC2.4 cell proliferation, antigen uptake, and maturation, as well as studying differences in DC2.4 exosome profiles following Toxoplasma gondii infection.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ DC2.4 Mouse Bone Marrow-Derived Dendritic Cell Line |
| Alternative Name | DC2.4 |
| Catalog Number | M3-1401 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Bone Marrow |
| Cell Characteristics | Adherent Growth; Few Suspended; Round Cells with Branched Processes |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | RPMI-1640 + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
