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NIH/3T3 Mouse Embryonic Fibroblast Cell Line with GFP
Introduction
The NIH/3T3 Mouse Embryonic Fibroblast Cell Line, established from NIH/Swiss mouse embryo cultures, is a continuous cell line. NIH/3T3 cells are highly sensitive to foci formation by sarcoma viruses and the replication of leukemia viruses, making them valuable for DNA transformation and transfection studies. This cell line is ectromelia virus (mousepox) negative. Due to its high transfection efficiency, this cell line is commonly used in laboratory research involving transfection and gene expression studies.
OriCell™ NIH/3T3 Mouse Embryonic Fibroblast Cell Line with GFP is generated by transducing NIH/3T3 cells with a recombinant lentiviral vector carrying the GFP gene, resulting in stable expression of green fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ NIH/3T3 Mouse Embryonic Fibroblast Cell Line with GFP |
| Alternative Name | NIH/3T3 with GFP |
| Catalog Number | M5-0303 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Embryo |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If GFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
