EL4 Mouse Lymphoma Cell Line
Introduction
The EL4 mouse lymphoma cell line was isolated from a lymphoma induced in C57BL mice by 9,10-dimethyl-1,2-benzanthracene (DMBA). The cells are resistant to 0.1 mM hydrocortisone and sensitive to 20 μg/mL phytohemagglutinin (PHA). Its subline, EL4.IL-2, is capable of producing high levels of IL-2.
OriCell™ EL4 Mouse Lymphoma Cell Line is widely used in lymphoma research, including the establishment of animal lymphoma models and the study of drug efficacy in these cells.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ EL4 Mouse Lymphoma Cell Line |
| Alternative Name | EL4 |
| Catalog Number | M5-0501 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Lymph Node |
| Cell Characteristics | Suspension Growth; Lymphoblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.