F344 Rat Cortical Astrocytes

Introduction

Astrocytes are star-shaped glial cells found in the brain and spinal cord. They play a crucial role in the formation of the blood-brain barrier (BBB), supplying nutrients to neural tissues, maintaining extracellular ionic balance, and facilitating the repair of brain and spinal cord injuries.

Cortical astrocytes are valuable for neurobiological research, including maintaining the extracellular environment of the central nervous system, supporting neuronal metabolism and neurotransmitter synthesis, and facilitating the research and development of new drugs for Parkinson's disease and Alzheimer's disease.

OriCell™ F344 Rat Cortical Astrocytes are isolated from the cerebral cortex of neonatal F344 rats.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma and endotoxin.
• Verified by cell recovery testing, with a post-thaw viability of > 80%.
• Verified by cell cycle analysis, with a doubling time < 72 h.
• Verified by immunofluorescence (IF): Positive for GFAP (≥ 80%); Negative for β-tubulin III (≤ 10%) and Galc (≤ 10%).

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Generally, cortical astrocytes have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Leveraging our extensive cell culture expertise and optimized culture systems, OriCell™ F344 Rat Cortical Astrocytes can be subcultured for more than 3 passages while maintaining their phenotypic integrity and meeting our rigorous quality control standards. However, we always recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ F344 Rat Cortical Astrocytes is (3–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.
• Refreezing of the cells after thawing is not recommended.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.