Introduction

Bone marrow mesenchymal stem cells are a type of pluripotent stem cells that exist in bone marrow stroma. Because of its strong value-added ability and immune regulation function, it is widely used in the fields of tissue engineering, cell therapy and gene therapy.

As a research hotspot, F344 Rat Bone Marrow Mesenchymal Stem Cells are widely used in regenerative medicine and tissue engineering, especially in the fields of bone, cardiovascular and nervous system diseases.

OriCell™ F344 Rat Bone Marrow Mesenchymal Stem Cells are extracted from healthy F344 rats bone marrow and have strong proliferation and multi-directional differentiation capabilities. After adherent culture, use lentiviral transduction with RFP gene to make the cells stably express RFP. It can be used as a cell model to study proliferation, aging, immunity, differentiation and transplantation.

When citing our products in academic journals, please indicate “OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44, CD90 are positive (>70%), while CD34, CD45, CD11b are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please reference "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Bone marrow mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell^TM F344 Rat Bone Marrow Mesenchymal Stem Cells With RFP can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of rat bone marrow mesenchymal stem cells is (2.5~4) ×10⁴ live cells/cm².   

Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.