Adipogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells
Introduction
The OriCell™ Adipogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells, carefully developed by the OriCell™ R&D team, contains a basic medium suitable for the growth of rat bone marrow mesenchymal stem cells, fetal bovine serum and various additives required for inducing cell differentiation.
This product is suitable for adipogenic induction and differentiation of rat bone marrow mesenchymal stem cells. Extensive cell culture data have demonstrated that it can reliably and efficiently induce these cells to differentiate into adipocytes.
When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."
Product information
| Components of Solution A | Catalog Number | Volume |
| OriCell™ Basal Medium For Cell Culture | BLDM-03011 | 177 mL |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 20 mL |
| OriCell™ Supplement For Rat Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation A-I | RAXMX-04031-a1 | 3 mL |
| OriCell™ Supplement For Rat Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation A-II | RAXMX-04031-a2 | 200 μL |
| Components of Solution B | Catalog Number | Volume |
| OriCell™ Basal Medium For Cell Culture | BLDM-03011 | 90 mL |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 10 mL |
| OriCell™ Supplement For Rat Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation B | RAXMX-04031-b | 200 μL |
| Other Components | Catalog Number | Volume |
| OriCell™ Oil Red O Solultion(pH=2.0~2.2) | OILR-10001 | 5 mL |
| OriCell™ 0.1% Gelatin Solution | GLT-11301 | 10 mL |
QC
- Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handing Principles
- Ensure that all equipment is kept clean and tidy.
- Standard operation method. Please operate according to the method described in the product manual.
- The ingredients should be properly stored in accordance with the storage conditions and used as soon as possible.
- If complete medium cannot be used in a short period of time, it should be prepared in batches according to the volume ratio of each component in the kit and stored in aliquots.
Product Stability and Storage Conditions
- All ingredients must be kept in dark place.
- The basal medium should be stored in a refrigerator at 4°C for a period of 1 year. Other components should be stored at -20°C for a period of 2 years.
- The prepared complete medium can be stored at 4°C for a period of 1 month. If the culture conditions are stable, the container has great sealing performance, and there is no alternation of hot and cold condition, the period of using can be appropriately extended, but not exceed 45 days.
- Please use all products before the expiration date. Expired ingredients may significantly affect the cell culture effect.
- Biocompatible Silica Nanoparticles-Insulin Conjugates for MesenchymalStem Cell Adipogenic Differentiation. 点击索取文献 ››
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- Evaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury. 点击索取文献 ››
- Plasmid-encapsulated polyethylene glycol-grafted polyethylenimine nanoparticles for gene delivery into rat mesenchymal stem cells. 点击索取文献 ››
- Does Injection of Metanephric Mesenchymal Cells Improve Renal Function in Rats. 点击索取文献 ››
- Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging. 点击索取文献 ››
- Mesenchymal Stem Cell and Chondrocyte Fates in a Multi-Shear Microdevice are Regulated by Yes-Associated Protein. 点击索取文献 ››
- Bone marrow-derived mesenchymal stem cells protect against cisplatin-induced acute kidney injury in rats by inhibiting cell apoptosis. 点击索取文献 ››
- Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds. 点击索取文献 ››
- Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration. 点击索取文献 ››
- Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering. 点击索取文献 ››
- The synergistic therapeutic effect of hepatocyte growth factor and granulocyte colony-stimulating factor on pulmonary hypertension in rats. 点击索取文献 ››
- Transplantation KCNMA1 modifed bone marrow-mesenchymal stem cell therapy for diabetes mellitus-induced erectile dysfunction. 点击索取文献 ››
- Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine. 点击索取文献 ››
- Dextran-coated fluorapatite crystals doped with Yb3þ/Ho3þ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo. 点击索取文献 ››
- Mesenchymal stem cells ameliorate_LPS-induced acute lung injury through_KGF promoting alveolar fluid clearance_of alveolar type II cells. 点击索取文献 ››
- Role of P2 7 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes. 点击索取文献 ››
- Mesenchymal stem cells ameliorate inflammatory cytokine‑induced impairment of AT‑II cells through a keratinocyte growth factor‑dependent PI3KAktmTOR signaling pathway. 点击索取文献 ››
- The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats. 点击索取文献 ››
- Cruciate ligament-derived mesenchymal stem cells a potential cell source for cartilage regeneration. 点击索取文献 ››
- The hepatocyte growth factor-expressing character is required for mesenchymal stem cells to protect the lung injured by lipopolysaccharide in vivo. 点击索取文献 ››
- The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo. 点击索取文献 ››
- Bone marrow mesenchymal stem cells attenuate 2,5-hexanedione-induced neuronal apoptosis through a NGF AKT-dependent pathway. 点击索取文献 ››
- Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling. 点击索取文献 ››
- Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats. 点击索取文献 ››
- Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats. 点击索取文献 ››
- Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model. 点击索取文献 ››
- Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells. 点击索取文献 ››
- Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity. 点击索取文献 ››
- Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo. 点击索取文献 ››
- Superoxide dismutase 3 facilitates the chondrogenesis of bone marrow-derived mesenchymal stem cells. 点击索取文献 ››
- Extracellular vesicles containing miR-146a attenuate experimental colitis by targeting TRAF6 and IRAK1. 点击索取文献 ››
- Osthole Stimulates Bone Formation, Drives Vascularization and Retards Adipogenesis to Alleviate Alcohol-Induced Osteonecrosis of the Femoral Head. 点击索取文献 ››
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Biocompatible Silica Nanoparticles-Insulin Conjugates for MesenchymalStem Cell Adipogenic Differentiation.
Bioconjug Chem 21:1673 (2010) IF = 4.513 Year 2010
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Differentiation Character of Adult Mesenchymal Stem Cells and Transfection of MSCs with Lentiviral Vectors.
J Huazhong Univ Sci Technolog Med Sci 30:687 (2010) IF = 0.834 Year 2010
-
Evaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury.
Brain Res 1391:24 (2011) IF = 2.843 Year 2011
-
Plasmid-encapsulated polyethylene glycol-grafted polyethylenimine nanoparticles for gene delivery into rat mesenchymal stem cells.
Int J Nanomedicine 6:843 (2011) IF = 4.383 Year 2011
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Does Injection of Metanephric Mesenchymal Cells Improve Renal Function in Rats.
Saudi J Kidney Dis Transpl 22:501 (2011) Year 2011
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Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging.
Eur Radiol 22:2328 (2012) IF = 4.014 Year 2012
-
Mesenchymal Stem Cell and Chondrocyte Fates in a Multi-Shear Microdevice are Regulated by Yes-Associated Protein.
Stem Cells Dev 22:2083 (2013) IF = 3.727 Year 2013
-
Bone marrow-derived mesenchymal stem cells protect against cisplatin-induced acute kidney injury in rats by inhibiting cell apoptosis.
Int J Mol Med 32:1262 (2013) IF = 2.088 Year 2013
-
Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds.
PLoS One PMID:23637803 (2013) IF = 3.234 Year 2013
-
Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration.
Cell Biol Int 37:826 (2013) Year 2013
-
Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering.
Biomed Mater 24770944 (2014) IF = 3.697 Year 2014
-
The synergistic therapeutic effect of hepatocyte growth factor and granulocyte colony-stimulating factor on pulmonary hypertension in rats.
Heart Vessels 29:520 (2014) IF = 2.065 Year 2014
-
Transplantation KCNMA1 modifed bone marrow-mesenchymal stem cell therapy for diabetes mellitus-induced erectile dysfunction.
Andrologia 46:479 (2014) IF = 1.63 Year 2014
-
Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine.
Stem Cell Res Ther PMID:25956503 (2015) IF = 4.963 Year 2015
-
Dextran-coated fluorapatite crystals doped with Yb3þ/Ho3þ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo.
Biomaterials PMID:25818450 (2015) IF = 8.557 Year 2015
-
Mesenchymal stem cells ameliorate_LPS-induced acute lung injury through_KGF promoting alveolar fluid clearance_of alveolar type II cells.
Eur Rev Med Pharmacol Sci 19:2368 (2015) IF = 1.213 Year 2015
-
Role of P2 7 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes.
Exp Cell Res 339:367 (2015) IF = 3.246 Year 2015
-
Mesenchymal stem cells ameliorate inflammatory cytokine‑induced impairment of AT‑II cells through a keratinocyte growth factor‑dependent PI3KAktmTOR signaling pathway.
Mol Med Rep 13: 3755 (2016) IF = 1.922 Year 2016
-
The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats.
Eur Spine J 24: 2411-24 (2016) IF = 2.634 Year 2015
-
Cruciate ligament-derived mesenchymal stem cells a potential cell source for cartilage regeneration.
Annals of Joint -2016 Year 2016
-
The hepatocyte growth factor-expressing character is required for mesenchymal stem cells to protect the lung injured by lipopolysaccharide in vivo.
Stem Cell Res Ther 7:66 (2016) IF = 4.963 Year 2016
-
The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo.
MEDIATORS OF INFLAMMATION DOI: 10.1155/2016/2347938 (2016) IF = 3.545 Year 2016
-
Bone marrow mesenchymal stem cells attenuate 2,5-hexanedione-induced neuronal apoptosis through a NGF AKT-dependent pathway.
Scientific Reports PMID:27703213 (2016) IF = 5.228 Year 2016
-
Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling.
INT J MOL MED 37:63-73 (2016) IF = 2.348 Year 2016
-
Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats.
Stem Cells Int 2017:2896874 (2017) IF = 3.54 Year 2017
-
Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats.
Stem Cells Int 2017:6761572 (2017) IF = 3.54 Year 2017
-
Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model.
Biol Blood Marrow Transplant 19:538-546 (2013) IF = 4.704 Year 2013
-
Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells.
Sci Rep 7:8447 (2017) IF = 4.259 Year 2017
-
Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity.
BioMed Research International DOI:10.1155/2017/9765843 IF = 2.476 Year 2017
-
Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo.
Biomed Pharmacother 106:1126-1134 (2018) IF = 3.457 Year 2018
-
Superoxide dismutase 3 facilitates the chondrogenesis of bone marrow-derived mesenchymal stem cells.
Biochem Biophys Res Commun PMID:30654942 (2019) IF = 2.559 Year 2019
-
Extracellular vesicles containing miR-146a attenuate experimental colitis by targeting TRAF6 and IRAK1.
Int Immunopharmacol 68:204-212 (2019) IF = 3.118 Year 2019
-
Osthole Stimulates Bone Formation, Drives Vascularization and Retards Adipogenesis to Alleviate Alcohol-Induced Osteonecrosis of the Femoral Head.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE PMID: 32135036 (2020) IF = 4.658 Year 2020