Osteogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells

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Introduction

OriCell™ Osteogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells, developed by the R&D team, contains a basic medium suitable for the growth of bone marrow mesenchymal stem cells, fetal bovine serum, and a supplement for osteogenic differentiation of bone marrow mesenchymal stem cells.

This product is suitable for osteogenic induction and differentiation of rat bone marrow mesenchymal stem cells. Extensive cell culture data have demonstrated that it can reliably and efficiently induce these cells to differentiate into osteoblasts.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."

Product Information

Components	Catalog Number	Volume
OriCell™ Basal Medium For Cell Culture	BLDM-03011	177 mL
OriCell™ Fetal Bovine Serum (Superior-Quality)	FBSSR-01021	20 mL
OriCell™ Supplement For Rat Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation	RAXMX-04021	3 mL
OriCell™ Alizarin Red S Solution (pH=5.1~5.3)	ALIR-10001	10 mL
OriCell™ 0.1% Gelatin Solution	GLT-11301	10 mL

QC

Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
Pass the detection of osmotic pressure and pH.
Pass the detection of product quality.

Please refer to "COA" for details.

General Handing Principles

Ensure that all equipment is kept clean and tidy.
Standard operation method. Please operate according to the method described in the product manual.
The ingredients should be properly stored in accordance with the storage conditions and used as soon as possible.
If complete medium cannot be used in a short period of time, it should be prepared in batches according to the volume ratio of each component in the kit and stored in aliquots.

Product Stability and Storage Conditions

All ingredients must be kept in dark place.
The basal medium should be stored in a refrigerator at 4°C for a period of 1 year. Other components should be stored at -20°C for a period of 2 years.
The prepared complete medium can be stored at 4°C for a period of 1 month. If the culture conditions are stable, the container has great sealing performance, and there is no alternation of hot and cold condition, the period of using can be appropriately extended, but not exceed 45 days.
Please use all products within the expiration date. Expired ingredients may significantly affect the cell culture effect.

Note: This product is intended for research use only and is not for diagnostic, therapeutic, clinical, household, or any other applications.

OriCell-RAXMX-90021-User-Manual.pdf View ››

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Plasmid-encapsulated polyethylene glycol-grafted polyethylenimine nanoparticles for gene delivery into rat mesenchymal stem cells.
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Does Injection of Metanephric Mesenchymal Cells Improve Renal Function in Rats.
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Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging.
Eur Radiol 22:2328 (2012) IF = 4.014 　Year 2012
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PLoS One PMID:24146754 (2013) IF = 3.234 　Year 2013
Control over the Gradient Dierentiation of Rat BMSCs on a PCL Membrane with Surface-Immobilized Alendronate Gradient.
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Mesenchymal Stem Cell and Chondrocyte Fates in a Multi-Shear Microdevice are Regulated by Yes-Associated Protein.
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Bone marrow-derived mesenchymal stem cells protect against cisplatin-induced acute kidney injury in rats by inhibiting cell apoptosis.
Int J Mol Med 32:1262 (2013) IF = 2.088 　Year 2013
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PLoS One PMID:23637803 (2013) IF = 3.234 　Year 2013
Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration.
Cell Biol Int 37:826 (2013) 　Year 2013
Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering.
Biomed Mater 24770944 (2014) IF = 3.697 　Year 2014
The synergistic therapeutic effect of hepatocyte growth factor and granulocyte colony-stimulating factor on pulmonary hypertension in rats.
Heart Vessels 29:520 (2014) IF = 2.065 　Year 2014
Transplantation KCNMA1 modifed bone marrow-mesenchymal stem cell therapy for diabetes mellitus-induced erectile dysfunction.
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Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine.
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Hydrostatic pressure promotes the proliferation and osteogenicchondrogenic differentiation of mesenchymal stem cells The roles of RhoA and Rac1.
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Investigating the Potential of Amnion-Based Scaffolds as a Barrier Membrane for Guided Bone Regeneration.
Langmuir 31:8642 (2015) IF = 4.457 　Year 2015
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The Potential Application of Pulsed Ultrasound on Bone Defect Repair via Developmental Engineering An In Vitro Study.
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Dorsal root ganglion neurons promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
Neural Regen Res 10:119 (2015) IF = 0.22 　Year 2015
Mesenchymal stem cells ameliorate inflammatory cytokine‑induced impairment of AT‑II cells through a keratinocyte growth factor‑dependent PI3KAktmTOR signaling pathway.
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Cruciate ligament-derived mesenchymal stem cells a potential cell source for cartilage regeneration.
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The hepatocyte growth factor-expressing character is required for mesenchymal stem cells to protect the lung injured by lipopolysaccharide in vivo.
Stem Cell Res Ther 7:66 (2016） IF = 4.963 　Year 2016
The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo.
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Bone marrow mesenchymal stem cells attenuate 2,5-hexanedione-induced neuronal apoptosis through a NGF AKT-dependent pathway.
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Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling.
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Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats.
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Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats.
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In vitro evaluation of electrospun silk fibroin/ nano-hydroxyapatite/BMP-2 scaffolds for bone regeneration.
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Low magnitude high frequency vibration promotes adipogenic differentiation of bone marrow stem cells via P38 MAPK signal.
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Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model.
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A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture.
Sci Rep 8:7693 (2018) IF = 4.259 　Year 2018
Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo.
Biomed Pharmacother 106:1126-1134 (2018) IF = 3.457 　Year 2018
BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling.
Stem Cell Res Ther 10:30 (2019) IF = 4.963 　Year 2019
Superoxide dismutase 3 facilitates the chondrogenesis of bone marrow-derived mesenchymal stem cells.
Biochem Biophys Res Commun PMID:30654942 (2019) IF = 2.559 　Year 2019
Extracellular vesicles containing miR-146a attenuate experimental colitis by targeting TRAF6 and IRAK1.
Int Immunopharmacol 68:204-212 (2019) IF = 3.118 　Year 2019
Hypoxia preconditioning promotes bone marrow mesenchymal stem cells survival by inducing HIF-1α in injured neuronal cells derived exosomes culture system.
Cell Death Dis 10:134 (2019) IF = 5.638 　Year 2019
A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration.
Advanced Science DOI:10.1002/advs.201900209 (2019) IF = 12.441 　Year 2019
Adhesive liposomes loaded onto an injectable, self-healing and antibacterial hydrogel for promoting bone reconstruction.
NPG Asia Materials 10.1038/s41427-019-0185-z (2019) IF = 8.052 　Year 2019
Arctigenin promotes bone formation involving PI3K/Akt/PPARγ signaling pathway.
Chemical Biology & Drug Design PMID: 31883507 (2020) IF = 2.256 　Year 2020
Artificial ligament made from silk protein/ Laponite hybrid fibers.
Acta Biomaterialia PMID: 32014583 (2020) IF = 6.638 　Year 2020
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