Wistar Rat Cortical Astrocytes

Introduction

Astrocytes are a type of stellate glial cell found in the brain and spinal cord. They play a crucial role in the formation of the blood-brain barrier. Astrocytes nourish neural tissue, maintain extracellular ion balance, and participate in the repair of brain and spinal cord injuries.

Cortical astrocytes are valuable for neurobiological research, including maintaining the extracellular environment of the central nervous system, supporting neuronal metabolism and neurotransmitter synthesis, and facilitating the research and development of new drugs for Parkinson's disease and Alzheimer's disease.

OriCell™ Wistar Rat Cortical Astrocytes are isolated from the cerebral cortex of newborn Wistar rats.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma and endotoxin.
• Pass the cell resuscitation test, the resuscitation survival rate is >50%.
• Pass the cell cycle detection, the doubling period is less than 72 h.
• Pass the immunofluorescence detection, it expresses GFAP (≥80%). But it does not express β-tubulin III ≤ 10%，Galc (≤ 10%).

Please reference "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions. Please operate according to the method described in the product manual, strictly control the variables, and do a controlled experiment.
• Use suitable and reliable consumables and reagents. This product needs to use a culture container suitable for the growth of adherent cells, and it is not recommended to reuse it. The reagents used must be validated, reliable, suitable for cell growth and have small batch-to-batch variation.
• Cortical astrocytes have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Based on the rich cell culture experience and excellent performance of the culture system, OriCell™ Wistar Rat Cortical Astrocytes can be passaged in vitro for more than 3 times and still maintain all indicators qualified. However, we always recommend using lower generation cells for scientific research as much as possible.
• OriCell™ Wistar Rat Cortical Astrocytes are usually inoculated at a density of (3~4) × 10⁴ viable cells/cm². The growth of this cell has a great relationship with the donor's own characteristics and the subsequent culture system.
• It is not recommended to cryopreserve the cells and reuse it.

Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.