SD Rat Tendon Stem Cells

Introduction

Rat tendon stem cells are a type of mesenchymal stem cell derived from rat tendon tissue, characterized by abilities such as self-renewal, multi-lineage differentiation, and colony formation. They are typically isolated from tendon tissue using a mixed enzymatic digestion method involving collagenase and neutral protease, and then cultured and expanded in a specialized tendon stem cell culture medium.

Tendon stem cells have the potential to differentiate into multiple lineages, including tendon cells (tenocytes), chondrocytes, osteocytes, and adipocytes. They play a crucial role in tendon injury repair by promoting the regeneration of tendon tissue and the restoration of its function. The normal self-renewal and differentiation of tendon stem cells are essential for maintaining the structure and function of tendons. Abnormal differentiation of these cells may lead to tendon disorders, such as chondrification, ossification, and adipogenesis within tendon tissue, which can adversely affect tendon healing.

Rat tendon stem cells, as a unique stem cell population, have become an important cellular resource in tendon tissue engineering and related disease treatment research due to their distinctive differentiation potential and key role in tendon repair.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44 and CD90 are positive (>70%), while CD34, CD45 and CD11b are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please refer to "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Rat tendon stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell™ SD Rat Tendon Stem Cells can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of rat tendon stem cells is (2.5~4) ×10⁴ live cells/cm².

Warm Notice: The cryopreservation solution of this product contains DMSO, which may pose potential risks. Please handle it with care.