SD Rat Skin Fibroblasts

Introduction

Fibroblasts are mesenchymal cells derived from the mesoderm. These cells have been widely used in cellular and molecular biology research due to their ease of culture. Fibroblasts secrete extracellular matrix components such as type I and type III collagen, and studies have shown significant heterogeneity among fibroblasts from different organs. During wound healing, dermal fibroblasts transition from a proliferative and migratory phenotype to a contractile and matrix-remodeling phenotype, while simultaneously secreting large amounts of hyaluronic acid to modulate the inflammatory response.

OriCell™ SD Rat Skin Fibroblasts are isolated from the skin tissue of neonatal rats (1–3 days old) and purified via the combined digestion of neutral protease and collagenase.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >90%.
• Cell identification: Positive for Vimentin expression (>90%) as confirmed by immunofluorescence (IF) staining.

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Since OriCell™ SD Rat Skin Fibroblasts have limited ability to proliferate in vitro, we recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ SD Rat Skin Fibroblasts is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.