SD Rat Neural Stem Cells

Introduction

Neural stem cells are pluripotent stem cells that can differentiate into various cell types of the nervous system, including neurons, astrocytes, oligodendrocytes, etc. Neural stem cells can be isolated from different regions of the mammalian brain, spinal cord, and other parts of the nervous system. Their capacity to rebuild neural circuits endows them with the potential for repairing brain tissue damage. Therefore, they hold extensive research value in animal models of neurodegenerative diseases, hereditary central nervous system disorders, stroke, and spinal cord injury.

OriCell™ SD Rat Neural Stem Cells are derived from the hippocampus of 14.5-day SD rats, cultured as suspended neurospheres, and cryopreserved at low passages. They can be used as a cell model to study proliferation, aging, immune responses, differentiation, and transplantation.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery testing, with a post-thaw viability of > 50%.
• Verified by cell cycle analysis, with a doubling time < 72 h.
• Verified by immunofluorescence: Positive for Nestin (> 75%); Negative for GFAP and Tubulin (< 10%).
• Proven differentiation potential into neurons, oligodendrocytes, astrocytes, etc.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for suspension growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Generally, neural stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Leveraging our extensive cell culture expertise and optimized culture systems, OriCell™ SD Rat Neural Stem Cells can be subcultured for more than 3 passages while meeting our rigorous quality control standards. However, we always recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ SD Rat Neural Stem Cells is (1.5–3) × 10⁵ viable cells/mL. Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which has potential risks. Please handle it carefully.