Introduction

Neural stem cells are pluripotent stem cells that can differentiate into a variety of cells of the nervous system, including neurons, astrocytes, oligodendrocytes, et al. Neural stem cells can be isolated from different regions of the mammalian brain and the spinal cord and other nervous systems. Neural stem cells have the ability to rebuild neural circuits, so they have the potential to treat brain tissue damage, and thus have extensive research value in animal models of neurodegenerative diseases, hereditary central nervous system diseases, stroke and spinal cord injury.

OriCell™ SD Rat Neural Stem Cells are derived from the brain tissue of 14.5 days old SD rats and are cultured in the form of suspended neurospheres. It was cryopreserved at low passages. It can be used as a cell model to study proliferation, aging, immunity, differentiation and transplantation.

When citing our products in academic journals, please indicate “OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the cell resuscitation test, the resuscitation survival rate is >50%.
• Pass the cell cycle detection, the doubling period is less than 72 h.
• Pass the immunofluorescence detection, it expresses Nestin (> 75%), but does not express GFAP and Tubulin (< 10%).
• Have good differentiation potential, can differentiate into neurons, oligodendrocytes, astrocytes, etc.

Please reference "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Neural stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Based on rich cell culture experience and excellent performance culture system, OriCell^TM SD Rat Neural Stem Cells With GFP can be passaged more than 3 times in vitro and still maintain all indicators qualified. However, we always recommend using lower generation cells for scientific research as much as possible.
• The usual inoculation density of neural stem cells in C57BL/6 mice is (1.5~3) × 10⁵ viable cells/mL. The growth of this cell has a great relationship with the donor's own characteristics and the subsequent culture system. We recommend subculture according to the actual growth conditions of each batch and each generation.

Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.