SD Rat Myocardial Cells

Introduction

OriCell™ SD Rat Myocardial Cells are isolated from rat heart tissue via synergistic digestion with trypsin and collagenase, followed by purification using the differential adhesion method. Once cultured in vitro, these cardiomyocytes retain key structural and functional properties while exhibiting characteristic spontaneous rhythmic contractions. This model offers distinct advantages, including experimental simplicity, quantifiability, high reproducibility, and free from neural and humoral interferences.

Owing to such unique characteristics, these cells are widely used to study the regulation of cardiac hypertrophy induced by non-hemodynamic factors, as well as biomechanics, apoptosis, receptor downregulation, ischemic preconditioning, and signaling pathways. In addition, they serve as valuable models for screening and evaluating the safety of new cardiac drugs, and for isolating key bioactive factors. Together, these applications are crucial for understanding cardiomyocyte physiological functions, drug responses, and pathological changes under various disease conditions.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >80%.
• Cell identification: Positive for α-actin expression (>90%) as confirmed by immunofluorescence (IF) staining.

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ SD Rat Myocardial Cells is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.