Introduction

Adipose-derived mesenchymal stem cells are a type of pluripotent stem cells that exist in adipose matrix. Because of its strong value-added ability and immune regulation function, it is widely used in the fields of tissue engineering, cell therapy and gene therapy.

As a research hotspot, C57BL/6 mouse adipose-derived mesenchymal stem cells are widely used in regenerative medicine and tissue engineering, especially in the fields of bone, cardiovascular and nervous system diseases.

OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells are taken from the inguinal fat of C57BL/6 mouse. These cells can express specific proteins of ADSCs and have strong proliferation and multidirectional differentiation capabilities. It can be used as a cell model to study proliferation, aging, immunity, differentiation and transplantation.

When citing our products in academic journals, please indicate “OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44 and Sca-1 are positive (>70%), while CD117 and CD31 are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please reference "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Adipose-derived mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of mouse adipose-derived mesenchymal stem cells is (2.5~4) ×10⁴ live cells/cm².