C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP

Introduction

Adipose-derived mesenchymal stem cells (ADMSCs) are a type of multipotent stem cell residing in adipose tissue. Owing to their strong proliferative capacity and immunomodulatory functions, ADMSCs are widely applied in tissue engineering, cell therapy, and gene therapy.

As a research hotspot, C57BL/6 mouse adipose-derived mesenchymal stem cells are widely used in regenerative medicine and tissue engineering, particularly in studies involving bone, cardiovascular, and nervous system disorders.

OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells are isolated from the inguinal fat of healthy C57BL/6 mice. These cells express characteristic markers of ADMSCs and exhibit robust proliferative capacity and multipotent differentiation potential. Following adherent culture, they can be stably transduced with a GFP-expressing lentiviral vector to achieve persistent GFP expression. They serve as a versatile cell model for investigating proliferation, aging, immune regulation, differentiation, and transplantation.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44 and Sca-1 are positive (>70%), while CD117 and CD31 are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please refer to "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Adipose-derived mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of mouse adipose-derived mesenchymal stem cells is (2.5~4) ×10⁴ live cells/cm².   

Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.