C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells

Introduction

Adipose-derived mesenchymal stem cells (ADMSCs) are a type of multipotent stem cell residing in adipose tissue. Owing to their strong proliferative capacity and immunomodulatory functions, ADMSCs are widely utilized in tissue engineering, cell therapy, and gene therapy.

As a focal point of current research, C57BL/6 mouse adipose-derived mesenchymal stem cells are widely used in regenerative medicine and tissue engineering, particularly in studies involving bone, cardiovascular, and nervous system disorders.

OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells are derived from the inguinal fat of healthy C57BL/6 mice. These cells express characteristic markers of ADMSCs, demonstrate robust proliferation, and possess multipotent differentiation potential. They serve as a premium cell model for research into proliferation, aging, immune regulation, differentiation, and transplantation.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery testing, with a post-thaw viability of >80%.
• Verified by cell cycle analysis, with a doubling time < 72 h.
• Verified by flow cytometry: Positive for CD29, CD44 and Sca-1 (> 70%); Negative for CD117 and CD31 (< 5%).
• Proven differentiation potential into osteoblasts, adipocytes, and chondrocytes.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Generally, ADMSCs have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Leveraging our extensive cell culture expertise and optimized culture systems, OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells can be subcultured for more than 5 passages while maintaining their phenotypic integrity and meeting our rigorous quality control standards. However, we always recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which has potential risks. Please handle it carefully.