ICR Mouse Embryonic Fibroblasts (Inactivated)

Introduction

Fibroblasts are cells that primarily secrete extracellular matrix and originate from mesenchymal cells in the embryonic mesoderm. Embryonic fibroblasts are commonly used as feeder cells for embryonic stem cell culture.

OriCell™ ICR Mouse Embryonic Fibroblasts (Inactivated) are derived from the trunk and limbs of 13.5-day ICR mouse embryos. These cells are cultured as monolayers, expanded to passage 1 (P1), then inactivated by γ-irradiation and cryopreserved. Mouse embryonic fibroblasts (MEFs) serve as feeder cells to support the culture of both mouse and human embryonic stem cells, helping to maintain their undifferentiated state.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery testing, with a post-thaw viability of >80%.
• Pass the test of cell ES supporting ability.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• MEF cells should be thawed one day prior to the thawing of embryonic stem cells.

Warm Notice: The cryopreservation medium of this product contains DMSO, which has potential risks. Please handle it carefully.