OriCell™ 129 Mouse Embryonic Stem Cells

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Introduction

Embryonic stem cells (ESC) are pluripotent stem cells derived from the inner cell mass of a blastocyst. It has the ability to differentiate into ectoderm, endoderm and mesoderm, and can differentiate into various types of cells. Unlike other stem cells, embryonic stem cells have the ability to proliferate indefinitely. The plasticity and unlimited proliferation of embryonic stem cells make it a hotspot in regenerative medicine and tissue engineering research.

129 mouse embryonic stem cells remain normal diploid after being expanded and cultured in vitro, expressing special markers of embryonic stem cells, and can form embryoid bodies (EB) when cultured in vitro, and teratomas can be formed in vivo experiments. 129 mouse embryonic stem cells are a powerful tool for basic and applied research in many fields, including development and regulation research, regenerative biology, and potential treatment methods. In addition, genetically modifying embryonic stem cells and introducing them into the mouse germline is a very effective method to obtain genetically modified mice.

OriCell™ 129 Mouse Embryonic Stem Cells are derived from the inner cell mass of the 129 mouse 3.5-day blastocyst stage, using OriCell™ mouse embryonic stem cell culture medium and cultured on γ-ray-treated MEF (mouse embryonic fibroblasts ).

When citing our products in academic journals, please indicate “OriCell™ + Catalog Number, from Cyagen Biosciences（Guangzhou）Inc.”

Product Information

Name	OriCell™ 129 Mouse Embryonic Stem Cells
Catalog Number	MUAES-01001
Amount of Cells	1×10⁶ cells/vial
Passage Number	P20
Storage at	Liquid Nitrogen（-196℃）

QC

Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
Pass the viability examination. The viable rates is higher than 50%.
The cell doubling time is less than 72 hours.
By immunofluorescence detection, it expresses Oct4, SSEA-1 and Nanog (≥ 90%), but does not express SSEA-3 and SSEA-4 (≤ 5%).

Please reference "COA" for details.

General Handing Principles

Ensure that all equipment is kept clean and tidy.
Please follow the instructions.
Use suitable and reliable consumables and reagents.
If using feeder cells for culture, it is recommended to use OriCell^TMICR Mouse Embryonic Fibroblasts(Inactivated)(MUIEF-01002).
If using serum-free and feeder-free culture conditions, it is recommended to use OriCell^TMSerum Free Medium For Mouse Embryonic Stem Cells (Type II, Feeder-free) (MUXES-90061).
Usually the inoculation density of OriCell^TMC57BL/6 mouse embryonic stem cells is (1~2) ×10⁴ live cells/cm².