Serum Free Medium For Mouse Embryonic Stem Cells (Type I, With Feeder Layer)

Introduction

OriCell™ Serum Free Medium For Mouse Embryonic Stem Cells (Type I, With Feeder Layer), carefully optimized by OriCell™ R&D team, contains a basal medium, selected serum-free supplements and others auxiliary ingredients.

This product is suitable for the serum-free culture of mouse embryonic stem cells (ES). It can maintain the good proliferation characteristics and highly undifferentiated totipotency of mouse embryonic stem cells in a serum-free and feeder-layer growth environment, and maintain correctness of the karyotype.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the detection of osmotic pressure and pH.
• Pass the detection of product quality.

Please refer to "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Standard operation method. Please operate according to the method described in the product manual, strictly control the variables, and do a controlled experiment.
• The ingredients should be properly stored in accordance with the storage conditions and used as soon as possible.
• If a complete set of medium cannot be used in a short period of time, it should be prepared in batches according to the volume ratio of each component in the kit and stored in aliquots.

Product Stability and Storage Conditions

• All ingredients must be kept in dark place.
• The basal medium should be stored in a refrigerator at 4°C for a period of 1 year. Other components should be stored at -20°C for a period of 2 years.
• The prepared complete medium can be stored at 4°C for a period of 1 month. If the culture conditions are stable, the container has great sealing performance, and there is no alternation of hot and cold condition, the period of using can be appropriately extended, but not exceed 45 days.
• Please use all products before the expiration date. Expired ingredients may significantly affect the cell culture effect.