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Neuronal Differentiation Medium For Mouse Embryonic Stem Cells
Introduction
Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of the blastocyst. They possess the capacity to differentiate into ectoderm, endoderm, and mesoderm lineages, and can further develop into various cell types. Unlike other types of stem cells, embryonic stem cells have the ability to proliferate indefinitely, exhibiting unlimited self-renewal capacity. Their pluripotency and infinite proliferative potential make them a focal point in regenerative medicine and tissue engineering research.
Neural regeneration holds great promise for patients suffering from neurodegenerative diseases or injuries, such as Parkinson's disease (PD), Alzheimer's disease (AD), Huntington's disease, and spinal cord injuries. However, expanding functional neurons for transplantation and scientific research remains a major challenge.
OriCell™ Neuronal Differentiation Medium For Mouse Embryonic Stem Cells effectively induces the differentiation of mouse embryonic stem cells into neurons. These differentiated cells are ideally suited for a wide range of downstream biological experiments.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Components of Solution A | Catalog Number | Volume | Storage |
| OriCell™ Basal Medium For Cell Culture A | BHDM-03011 | 178 mL | 4 ℃ |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 20 mL | -20 ℃ |
| OriCell™ Supplements For Mouse ES Neuronal Differentiation A | MUXES-04081-a | 2 mL | -20 ℃ |
| Components of Solution B | Catalog Number | Volume | Storage |
| OriCell™ Basal Medium For Cell Culture B | BNRO-03011 | 98 mL | 4 ℃ |
| OriCell™ Supplements For Mouse ES Neuronal Differentiation B | MUXES-04081-b | 2 mL | -20 ℃ |
| Other Components | Catalog Number | Volume | Storage |
| Poly-L-Lysine Solution | PLLY-10001 | 200 μL | -20 ℃ |
| Gelatin | GLT-11301 | 50 mL | 4 ℃ |
QC
- Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
- Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.
Product Stability and Storage Conditions
- All components must be stored away from light.
- The basal medium and gelatin have a shelf life of 1 year and should be stored at 4 °C. Fetal bovine serum, Supplement A, and Supplement B have a shelf life of 2 years and should be stored at −20 °C. The remaining component (Poly-L-Lysine Solution) has a shelf life of 1 year and should be stored at −20 °C.
- Once prepared, the medium has a shelf life of 1 month when stored at 4 °C. The shelf life may be extended up to a maximum of 45 days, provided that culture conditions remain stable, the container is properly sealed, and repeated temperature fluctuations are avoided.
- Use all components before the expiration dates. Expired components may severely compromise culture performance.
