C57BL/6 Mouse Renal Tubular Epithelial Cells

Introduction

The renal tubule is a slender epithelial tubule connected to the parietal layer of Bowman's capsule in the kidney. It is mainly responsible for reabsorption and secretion. Based on differences in morphological structure, location, and function, the renal tubule can be divided into three parts: the proximal tubule, the loop of Henle, and the distal tubule. The proximal tubule can be further subdivided into the straight and convoluted segments. The convoluted segment, also known as the proximal convoluted tubule (PCT), is located in the cortical labyrinth and exhibits extensive coiling around the renal corpuscle. The convoluted segment of the distal tubule, known as the distal convoluted tubule (DCT), is also situated in the cortical labyrinth. Renal tubular epithelial cells (RTECs) form the epithelial lining of the renal tubule. The isolation and culture of these cells are essential techniques in the study of kidney diseases.

OriCell™ C57BL/6 Mouse Renal Tubular Epithelial Cells are isolated from the renal cortex of C57BL/6 mice via collagenase digestion, followed by purification and expansion in specialized epithelial cell culture medium.

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Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >80%.
• Cell characterization: Positive for Cytokeratin 18 (CK-18) expression (>90%) as confirmed by immunofluorescence (IF) staining.

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Since OriCell™ C57BL/6 Mouse Renal Tubular Epithelial Cells have limited ability to proliferate in vitro (typically up to 2 passages), we recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ C57BL/6 Mouse Renal Tubular Epithelial Cells is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.