Introduction

Bone marrow mesenchymal stem cells are a type of pluripotent stem cells that exist in bone marrow stroma. Because of its strong proliferation ability and immune regulation function, it is widely used in the fields of tissue engineering, cell therapy and gene therapy.

As a research hotspot, C57BL/6 mouse bone marrow mesenchymal stem cells are widely used in regenerative medicine and tissue engineering, especially in the fields of bone, cardiovascular and nervous system diseases.

OriCell™ C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells are extracted from healthy C57BL/6 mouse bone marrow and have strong proliferation and multi-directional differentiation capabilities. After adherent culture, use lentiviral transduction with GFP gene to make the cells stably express GFP. It can be used as a cell model to study proliferation, aging, immunity, differentiation and transplantation.

When citing our products in academic journals, please indicate “OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44 and Sca-1 are positive (>70%), while CD117 and CD31 are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please reference "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions.
• Use suitable and reliable consumables and reagents.
• Bone marrow mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell^TM C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of mouse bone marrow mesenchymal stem cells is (2.5~4) ×10⁴ live cells/cm².   

Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.