C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP

Introduction

Bone marrow mesenchymal stem cells (BMSCs) are a type of multipotent stem cell residing in the bone marrow stroma. Due to their strong proliferative capacity and immunomodulatory properties, BMSCs are widely applied in tissue engineering, cell therapy, and gene therapy.

As a research hotspot, C57BL/6 mouse bone marrow mesenchymal stem cells are commonly used in regenerative medicine and tissue engineering, particularly in studies involving bone, cardiovascular, and nervous system disorders.

OriCell™ C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells are isolated from the bone marrow of healthy C57BL/6 mice. These cells exhibit robust proliferation and multipotent differentiation potential. Following adherent culture, they can be stably transduced with a GFP-expressing lentiviral vector to achieve persistent GFP expression. They serve as a versatile cell model for investigating proliferation, aging, immune regulation, differentiation, and transplantation.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery testing, with a post-thaw viability of >80%.
• Verified by cell cycle analysis, with a doubling time < 72 h.
• Verified by flow cytometry: Positive for CD29, CD44 and Sca-1 (> 70%); Negative for CD117 and CD31 (< 5%).
• Proven differentiation potential into osteoblasts, adipocytes, and chondrocytes.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Generally, BMSCs have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Leveraging our extensive cell culture expertise and optimized culture systems, OriCell™ C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP can be subcultured for more than 5 passages while maintaining their phenotypic integrity and meeting our rigorous quality control standards. However, we always recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which has potential risks. Please handle it carefully.