Complete Medium For Mouse Bone Marrow Mesenchymal Stem Cells
Introduction
OriCell™ Complete Medium For Mouse Bone Marrow Mesenchymal Stem Cells is specially formulated and developed by our R&D team. It consists of an optimized basal medium, premium fetal bovine serum (FBS), and essential growth supplements, all tailored for mouse bone marrow mesenchymal stem cells.
Extensive cell culture data have demonstrated that this product supports the long-term maintenance and healthy growth of mouse BMSCs in vitro, enabling them to retain their multi-lineage differentiation potential—including osteogenic, adipogenic, and chondrogenic lineages—even after multiple passages.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Components | Catalog Number | Volume | Storage |
| OriCell™ Basal Medium For Cell Culture | BDMF-03011 | 450 mL | 4 °C |
| OriCell™ Fetal Bovine Serum + Culture Supplement (For Mouse Bone Marrow Mesenchymal Stem Cells) | MUXMX-05001 | 50 mL | -20 °C |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
- Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.
Product Stability and Storage Conditions
- All components must be stored away from light.
- The basal medium has a shelf life of 1 year and should be stored at 4 °C. Other component has a shelf life of 2 years and should be stored at -20 °C.
- Once prepared, the medium has a shelf life of 1 month when stored at 4 °C. The shelf life may be extended up to a maximum of 45 days, provided that culture conditions remain stable, the container is properly sealed, and repeated temperature fluctuations are avoided.
- Use all components before the expiration dates. Expired components may severely compromise culture performance.
- Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium. 点击索取文献 ››
- Arterially transplanted mesenchymal stem cells in a mouse reversible unilateral ureteral obstruction model: in vivo bioluminescence imaging and effects on renal fibrosis. 点击索取文献 ››
- The immunomodulatory effect of bone marrow stromal cells(BMSCs) on interleukin(IL)-23/IL-17-mediated ischemic stroke in mice. 点击索取文献 ››
- Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Laser-Induced Retinal Injury in Mice. 点击索取文献 ››
- MiR-335-5p promotes chondrogenesis in mouse mesenchymal stem cells and is regulated through two positive feedback loops. 点击索取文献 ››
- Mesenchymal stem cells ameliorate rhabdomyolysis-induced acute kidney injury via the activation of M2 macrophages. 点击索取文献 ››
- Coregulator CCAR1 positively regulates adipocyte differentiation through the glucocorticoid signaling pathway. 点击索取文献 ››
- Allogeneic compact bone-derived mesenchymal stem cell transplantation increases survival of mice exposed to lethal total body irradiation: a potential immunological mechanism. 点击索取文献 ››
- Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice. 点击索取文献 ››
- Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system. 点击索取文献 ››
- Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing. 点击索取文献 ››
- CCR2 Positive Exosome Released by Mesenchymal Stem Cells Suppresses Macrophage Functions and Alleviates IschemiaReperfusion-Induced Renal Injury. 点击索取文献 ››
- Biological Membrane-Packed Mesenchymal Stem Cells Treat Acute Kidney Disease by Ameliorating MitochondrialRelated Apoptosis. 点击索取文献 ››
- MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing. 点击索取文献 ››
- Bre Enhances Osteoblastic Differentiation by Promoting the Mdm2-Mediated Degradation of p53. 点击索取文献 ››
- Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells. 点击索取文献 ››
- Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions. 点击索取文献 ››
- Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB. 点击索取文献 ››
- Evaluation of the osteo-inductive potential of hollow three-dimensional magnesium-strontium substitutes for the bone grafting application. 点击索取文献 ››
- Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system. 点击索取文献 ››
- Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs. 点击索取文献 ››
- The Optimal Intervention Time of Bone Marrow Mesenchymal Stem Cells in Ameliorating Cardiac Fibrosis Induced by Viral Myocarditis: A Randomized Controlled Trial in Mice. 点击索取文献 ››
- SCAFFOLDS WITH INTEGRATED PROPERTIES AND APPLICATIONS IN CELL TRANSPLANTATION. 点击索取文献 ››
- Osteopontin activates mesenchymal stem cells to repair skin wound. 点击索取文献 ››
- Improved osteogenesis by HVEM‐expressing allogenic bone marrow‐derived mesenchymal stem cells in an immune activation condition and mouse femoral defect model. 点击索取文献 ››
- C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction. 点击索取文献 ››
- Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells. 点击索取文献 ››
- Loading of Metal Isotope-Containing Intercalators for Mass Cytometry-Based High-Throughput Quantitation of Exosome Uptake at the Single-Cell Level. 点击索取文献 ››
-
Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium.
Exp Biol Med (Maywood) 237:678 (2012) IF = 2.165 Year 2012
-
Arterially transplanted mesenchymal stem cells in a mouse reversible unilateral ureteral obstruction model: in vivo bioluminescence imaging and effects on renal fibrosis.
Chin Med J (Engl) 126:1890 (2013) Year 2013
-
The immunomodulatory effect of bone marrow stromal cells(BMSCs) on interleukin(IL)-23/IL-17-mediated ischemic stroke in mice.
J Neuroimmunol 257:28 (2013) IF = 2.786 Year 2013
-
Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Laser-Induced Retinal Injury in Mice.
Int J Mol Sci 15:9372 (2014) IF = 2.862 Year 2014
-
MiR-335-5p promotes chondrogenesis in mouse mesenchymal stem cells and is regulated through two positive feedback loops.
J Bone Miner Res 29:1575 (2014) IF = 6.589 Year 2014
-
Mesenchymal stem cells ameliorate rhabdomyolysis-induced acute kidney injury via the activation of M2 macrophages.
Stem Cell Res Ther 5:80 (2014) IF = 4.963 Year 2014
-
Coregulator CCAR1 positively regulates adipocyte differentiation through the glucocorticoid signaling pathway.
J Biol Chem 289:17078 (2014) IF = 4.6 Year 2014
-
Allogeneic compact bone-derived mesenchymal stem cell transplantation increases survival of mice exposed to lethal total body irradiation: a potential immunological mechanism.
Chin Med J (Engl) 127:475 (2014) Year 2014
-
Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice.
PLoS One PMID:25693202 (2015) IF = 3.234 Year 2015
-
Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
BRAIN BEHAV IMMUN -2015 IF = 5.899 Year 2015
-
Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing.
Biomaterials 53:659 (2015) IF = 8.557 Year 2015
-
CCR2 Positive Exosome Released by Mesenchymal Stem Cells Suppresses Macrophage Functions and Alleviates IschemiaReperfusion-Induced Renal Injury.
Stem Cells International 1240301 (2016) IF = 3.687 Year 2016
-
Biological Membrane-Packed Mesenchymal Stem Cells Treat Acute Kidney Disease by Ameliorating MitochondrialRelated Apoptosis.
Scientific Reports 7:41136 (2017) IF = 4.259 Year 2017
-
MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing.
RSC Advances 22998-23010 (2017) IF = 3.108 Year 2017
-
Bre Enhances Osteoblastic Differentiation by Promoting the Mdm2-Mediated Degradation of p53.
STEM CELLS 35:1760–1772 (2017) IF = 5.599 Year 2017
-
Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells.
Nucleic Acids Research 45:5930–5944 (2017) IF = 10.162 Year 2017
-
Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions.
Med Sci Monit 23:3451-3460 (2017) IF = 1.585 Year 2017
-
Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.
PLoS ONE 12:e0179248 IF = 2.806 Year 2017
-
Evaluation of the osteo-inductive potential of hollow three-dimensional magnesium-strontium substitutes for the bone grafting application.
Materials Science and Engineering C 73:347-356 (2017) IF = 4.164 Year 2017
-
Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system.
Mol Med Rep 16:9473-9479 (2017) IF = 1.922 Year 2017
-
Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs.
BMC Musculoskeletal Disorders 18:423 (2017) IF = 1.739 Year 2017
-
The Optimal Intervention Time of Bone Marrow Mesenchymal Stem Cells in Ameliorating Cardiac Fibrosis Induced by Viral Myocarditis: A Randomized Controlled Trial in Mice.
Stem Cells International -2017 IF = 3.54 Year 2017
-
SCAFFOLDS WITH INTEGRATED PROPERTIES AND APPLICATIONS IN CELL TRANSPLANTATION.
Dissertation -2017 Year 2017
-
Osteopontin activates mesenchymal stem cells to repair skin wound.
PLoS One 12 (9):e0185346 (2017) IF = 2.806 Year 2017
-
Improved osteogenesis by HVEM‐expressing allogenic bone marrow‐derived mesenchymal stem cells in an immune activation condition and mouse femoral defect model.
Tissue Engineering 24:1167-1178 (2017) IF = 3.485 Year 2017
-
C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction.
Cell Death & Disease 10:530 (2019) IF = 5.959 Year 2019
-
Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE PMID: 31782262 (2019) IF = 4.658 Year 2019
-
Loading of Metal Isotope-Containing Intercalators for Mass Cytometry-Based High-Throughput Quantitation of Exosome Uptake at the Single-Cell Level.
Biomaterials DOI: 10.1016/j.biomaterials.2020.120152 IF = 10.273 Year 2020