Adipogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells
Introduction
The OriCell™ Adipogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells, carefully developed by the OriCell™ R&D team, contains a basic medium suitable for the growth of mouse bone marrow mesenchymal stem cells, fetal bovine serum and various additives required for inducing cell differentiation.
This product is suitable for adipogenic induction and differentiation of mouse bone marrow mesenchymal stem cells. Extensive cell culture data have demonstrated that it can reliably and efficiently induce these cells to differentiate into adipocytes.
When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."
Product Information
| Components of Solution A | Catalog Number | Volume |
| OriCell™ Basal Medium For Cell Culture | BLDM-03011 | 177 mL |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 20 mL |
| OriCell™ Supplements For Mouse Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation A-I | MUXMX-04031-a1 | 3 mL |
| OriCell™ Supplements For Mouse Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation A-II | MUXMX-04031-a2 | 200 μL |
| Components of Solution B | Catalog Number | Volume |
| OriCell™ Basal Medium For Cell Culture | BLDM-03011 | 90 mL |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 10 mL |
| OriCell™ Supplements For Mouse Bone Marrow Mesenchymal Stem Cells Adipogenic Differentiation B | MUXMX-04031-b | 200 μL |
| Other Components | Catalog Number | Volume |
| OriCell™ Oil Red O Solution(pH=2.0~2.2) | OILR-10001 | 5 mL |
| OriCell™ 0.1% Gelatin Solution | GLT-11301 | 10 mL |
QC
- Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handing Principles
- Ensure that all equipment is kept clean and tidy.
- Standard operation method. Please operate according to the method described in the product manual.
- The ingredients should be properly stored in accordance with the storage conditions and used as soon as possible.
- If complete medium cannot be used in a short period of time, it should be prepared in batches according to the volume ratio of each component in the kit and stored in aliquots.
Product Stability and Storage Conditions
- All ingredients must be kept in dark place.
- The basal medium should be stored in a refrigerator at 4°C for a period of 1 year. Other components should be stored at -20°C for a period of 2 years.
- The prepared complete medium can be stored at 4°C for a period of 1 month. If the culture conditions are stable, the container has great sealing performance, and there is no alternation of hot and cold condition, the period of using can be appropriately extended, but not exceed 45 days.
- Please use all products before the expiration date. Expired ingredients may significantly affect the cell culture effect.
- Implantation of biomaterial as an efficient method to harvest mesenchymal stem cells. 点击索取文献 ››
- Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium. 点击索取文献 ››
- Activation of canonical wnt pathway promotes differentiation of mouse bone marrow-derived MSCs into type II alveolar epithelial cells, confers resistance to oxidative stress, and promotes their migration to injured lung tissue in vitro. 点击索取文献 ››
- Migration of CXCR4 Gene‐Modified Bone Marrow‐Derived Mesenchymal Stem Cells to the Acute Injured Kidney. 点击索取文献 ››
- Hematopoietic recovery folowing chemtheraphy is improved by BADGE-induced inhibition of adipogenesis. 点击索取文献 ››
- Allogeneic compact bone-derived mesenchymal stem cell transplantation increases survival of mice exposed to lethal total body irradiation: a potential immunological mechanism. 点击索取文献 ››
- Leukemia Propagating Cells Rebuild an Evolving Niche in Response to Therapy. 点击索取文献 ››
- Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse. 点击索取文献 ››
- Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice. 点击索取文献 ››
- Olfactory ecto-mesenchymal stem cells possess immunoregulatory function and suppress autoimmune arthritis. 点击索取文献 ››
- Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon. 点击索取文献 ››
- Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells. 点击索取文献 ››
- Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions. 点击索取文献 ››
- Mesenchymal Stem Cells Promote Diabetic Corneal Epithelial Wound Healing Through TSG-6–Dependent Stem Cell Activation and Macrophage Switch. 点击索取文献 ››
- Mechano-signal transduction in mesenchymal stem cells induces prosaposin secretion to drive the 1 proliferation of breast cancer cells. 点击索取文献 ››
- Improved osteogenesis by HVEM‐expressing allogenic bone marrow‐derived mesenchymal stem cells in an immune activation condition and mouse femoral defect model. 点击索取文献 ››
- Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells. 点击索取文献 ››
- Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. 点击索取文献 ››
- Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3. 点击索取文献 ››
- C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction. 点击索取文献 ››
- Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells. 点击索取文献 ››
- Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization. 点击索取文献 ››
- Extracellular vesicles from bone marrow mesenchymal stem cells alleviate acute rejection injury after liver transplantation by carrying miR-22-3p and inducing M2 polarization of Kupffer cells. 点击索取文献 ››
-
Implantation of biomaterial as an efficient method to harvest mesenchymal stem cells.
Exp Biol Med (Maywood) 236:1477 (2011) IF = 2.165 Year 2011
-
Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium.
Exp Biol Med (Maywood) 237:678 (2012) IF = 2.165 Year 2012
-
Activation of canonical wnt pathway promotes differentiation of mouse bone marrow-derived MSCs into type II alveolar epithelial cells, confers resistance to oxidative stress, and promotes their migration to injured lung tissue in vitro.
J Cell Physiol 228:1270 (2013) IF = 3.874 Year 2013
-
Migration of CXCR4 Gene‐Modified Bone Marrow‐Derived Mesenchymal Stem Cells to the Acute Injured Kidney.
J Cell Biochem 114:2677 (2013) IF = 3.263 Year 2013
-
Hematopoietic recovery folowing chemtheraphy is improved by BADGE-induced inhibition of adipogenesis.
Int J Hematol 97:58 (2013) IF = 1.918 Year 2013
-
Allogeneic compact bone-derived mesenchymal stem cell transplantation increases survival of mice exposed to lethal total body irradiation: a potential immunological mechanism.
Chin Med J (Engl) 127:475 (2014) Year 2014
-
Leukemia Propagating Cells Rebuild an Evolving Niche in Response to Therapy.
Cancer Cell 25:778 (2014) IF = 23.523 Year 2014
-
Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse.
ScientificWorldJournal 24999489 (2014) Year 2014
-
Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice.
PLoS One PMID:25693202 (2015) IF = 3.234 Year 2015
-
Olfactory ecto-mesenchymal stem cells possess immunoregulatory function and suppress autoimmune arthritis.
Cell Mol Immunol PMID:26388237 (2015) IF = 4.112 Year 2015
-
Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon.
Scientific Reports 7:44498 (2017) IF = 4.259 Year 2017
-
Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells.
Nucleic Acids Research 45:5930–5944 (2017) IF = 10.162 Year 2017
-
Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions.
Med Sci Monit 23:3451-3460 (2017) IF = 1.585 Year 2017
-
Mesenchymal Stem Cells Promote Diabetic Corneal Epithelial Wound Healing Through TSG-6–Dependent Stem Cell Activation and Macrophage Switch.
Investigative Ophthalmology & Visual Science 5810:4064-4074 (2017) IF = 3.303 Year 2017
-
Mechano-signal transduction in mesenchymal stem cells induces prosaposin secretion to drive the 1 proliferation of breast cancer cells.
Cancer Research 77:6179-6189 (2017) IF = 9.122 Year 2017
-
Improved osteogenesis by HVEM‐expressing allogenic bone marrow‐derived mesenchymal stem cells in an immune activation condition and mouse femoral defect model.
Tissue Engineering 24:1167-1178 (2017) IF = 3.485 Year 2017
-
Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells.
Stem Cell Res Ther 1:31 (2019) IF = 4.963 Year 2019
-
Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow.
Exp Ther Med 16:5171-5177 (2019) IF = 1.41 Year 2019
-
Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3.
Experimental Cell Research PMID:31170401 (2019) IF = 3.309 Year 2019
-
C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction.
Cell Death & Disease 10:530 (2019) IF = 5.959 Year 2019
-
Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE PMID: 31782262 (2019) IF = 4.658 Year 2019
-
Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization.
Journal of Neuroinflammation DOI: 10.1186/s12974-020-1726-7 (2020) IF = 5.7 Year 2020
-
Extracellular vesicles from bone marrow mesenchymal stem cells alleviate acute rejection injury after liver transplantation by carrying miR-22-3p and inducing M2 polarization of Kupffer cells.
JOURNAL OF GENE MEDICINE IF = 4.152 Year 2023