Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP
Introduction
Bone marrow mesenchymal stem cells (BMSCs) are a type of multipotent stem cell residing in the bone marrow stroma. Due to their strong proliferative capacity and immunomodulatory properties, BMSCs are widely applied in tissue engineering, cell therapy, and gene therapy.
As a research hotspot, Balb/c mouse bone marrow mesenchymal stem cells are commonly used in regenerative medicine and tissue engineering, particularly in studies involving bone, cardiovascular, and nervous system disorders.
OriCell™ Balb/c Mouse Bone Marrow Mesenchymal Stem Cells are isolated from the bone marrow of healthy Balb/c mice. These cells exhibit robust proliferation and multipotent differentiation potential. Following adherent culture, they can be stably transduced with a GFP-expressing lentiviral vector to achieve persistent GFP expression. They serve as a versatile cell model for investigating proliferation, aging, immune regulation, differentiation, and transplantation.
When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."
Product Information
| Name | OriCell™ Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP |
| Catalog Number | MUCMX-01101 |
| Amount of Cells | 1×106 cells/vial |
| Passage Number | P6 |
| Storage at | Liquid Nitrogen (-196℃) |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Pass the viability examination. The viable rates is higher than 80%.
- The cell doubling time is less than 72 hours.
- Flow cytometry showed that CD29, CD44 and Sca-1 are positive (>70%), while CD117 and CD31 are negative (<5%).
- The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.
Please refer to "COA" for details.
General Handing Principles
- Ensure that all equipment is kept clean and tidy.
- Please follow the instructions.
- Use suitable and reliable consumables and reagents.
- Bone marrow mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell™ Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
- Usually the inoculation density of mouse bone marrow mesenchymal stem cells is (2.5~4) ×104 live cells/cm2.
Warm Notice: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.
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Arterially transplanted mesenchymal stem cells in a mouse reversible unilateral ureteral obstruction model: in vivo bioluminescence imaging and effects on renal fibrosis.
Chin Med J (Engl) 126:1890 (2013) Year 2013