Osteogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells
Introduction
OriCell™ Osteogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells is specially formulated and developed by our R&D team. It consists of an optimized basal medium, fetal bovine serum (FBS) and essential supplements, tailored to induce the osteogenic differentiation of mouse bone marrow mesenchymal stem cells (BMSCs).
Extensive data have demonstrated that this medium consistently facilitates the stable and efficient differentiation of these cells into osteoblasts.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Components | Catalog Number | Volume | Storage |
| OriCell™ Basal Medium For Cell Culture | BLDM-03011 | 177 mL | 4 °C |
| OriCell™ Fetal Bovine Serum (Superior-Quality) | FBSSR-01021 | 20 mL | -20 °C |
| OriCell™ Supplements For Mouse Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation | MUXMX-04021 | 3 mL | -20 °C |
| Alizarin Red S Solution (pH = 5.1 ~ 5.3) | ALIR-10001 | 10 mL | 4 °C |
| Gelatin | GLT-11301 | 10 mL | 4 °C |
QC
- Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
- Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.
Product Stability and Storage Conditions
- All components must be stored away from light.
- The basal medium, Alizarin Red S solution and gelatin have a shelf life of 1 year and should be stored at 4 °C. Other components have a shelf life of 2 years and should be stored at -20 °C.
- Once prepared, the medium has a shelf life of 1 month when stored at 4 °C. The shelf life may be extended up to a maximum of 45 days, provided that culture conditions remain stable, the container is properly sealed, and repeated temperature fluctuations are avoided.
- Use all components before the expiration dates. Expired components may severely compromise culture performance.
- Implantation of biomaterial as an efficient method to harvest mesenchymal stem cells. 点击索取文献 ››
- Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium. 点击索取文献 ››
- Activation of canonical wnt pathway promotes differentiation of mouse bone marrow-derived MSCs into type II alveolar epithelial cells, confers resistance to oxidative stress, and promotes their migration to injured lung tissue in vitro. 点击索取文献 ››
- Migration of CXCR4 Gene‐Modified Bone Marrow‐Derived Mesenchymal Stem Cells to the Acute Injured Kidney. 点击索取文献 ››
- Leukemia Propagating Cells Rebuild an Evolving Niche in Response to Therapy. 点击索取文献 ››
- Bone marrow mesenchymal stem cells promote the repair of islets from diabetic mice through paracrine actions. 点击索取文献 ››
- Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse. 点击索取文献 ››
- Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice. 点击索取文献 ››
- Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon. 点击索取文献 ››
- Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells. 点击索取文献 ››
- Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions. 点击索取文献 ››
- Mesenchymal Stem Cells Promote Diabetic Corneal Epithelial Wound Healing Through TSG-6–Dependent Stem Cell Activation and Macrophage Switch. 点击索取文献 ››
- Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs. 点击索取文献 ››
- Mechano-signal transduction in mesenchymal stem cells induces prosaposin secretion to drive the 1 proliferation of breast cancer cells. 点击索取文献 ››
- Intramembranous ossification and endochondral ossification are impaired differently between glucocorticoid-induced osteoporosis and estrogen deficiency-induced osteoporosis. 点击索取文献 ››
- Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells. 点击索取文献 ››
- Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. 点击索取文献 ››
- Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3. 点击索取文献 ››
- C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction. 点击索取文献 ››
- Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells. 点击索取文献 ››
- Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization. 点击索取文献 ››
-
Implantation of biomaterial as an efficient method to harvest mesenchymal stem cells.
Exp Biol Med (Maywood) 236:1477 (2011) IF = 2.165 Year 2011
-
Novel vascular endothelial growth factor gene delivery system-manipulated mesenchymal stem cells repair infarcted myocardium.
Exp Biol Med (Maywood) 237:678 (2012) IF = 2.165 Year 2012
-
Activation of canonical wnt pathway promotes differentiation of mouse bone marrow-derived MSCs into type II alveolar epithelial cells, confers resistance to oxidative stress, and promotes their migration to injured lung tissue in vitro.
J Cell Physiol 228:1270 (2013) IF = 3.874 Year 2013
-
Migration of CXCR4 Gene‐Modified Bone Marrow‐Derived Mesenchymal Stem Cells to the Acute Injured Kidney.
J Cell Biochem 114:2677 (2013) IF = 3.263 Year 2013
-
Leukemia Propagating Cells Rebuild an Evolving Niche in Response to Therapy.
Cancer Cell 25:778 (2014) IF = 23.523 Year 2014
-
Bone marrow mesenchymal stem cells promote the repair of islets from diabetic mice through paracrine actions.
Mol Cell Endocrinol 388:41 (2014) IF = 4.405 Year 2014
-
Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse.
ScientificWorldJournal 24999489 (2014) Year 2014
-
Inhibited Wnt signaling causes age-dependent abnormalities in the bone matrix mineralization in the Apert syndrome FGFR2(S252W/+) mice.
PLoS One PMID:25693202 (2015) IF = 3.234 Year 2015
-
Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon.
Scientific Reports 7:44498 (2017) IF = 4.259 Year 2017
-
Identification of miRNA-7 by genome-wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells.
Nucleic Acids Research 45:5930–5944 (2017) IF = 10.162 Year 2017
-
Peroxisome Proliferator-Activated Receptor-g Coactivator-1a (PGC-1a) Regulates the Expression of B-Cell Lymphoma/Leukemia-2 (Bcl-2) and Promotes the Survival of Mesenchymal Stem Cells (MSCs) via PGC-1a/ERRa Interaction in the Absence of Serum, Hypoxia, and High Glucose Conditions.
Med Sci Monit 23:3451-3460 (2017) IF = 1.585 Year 2017
-
Mesenchymal Stem Cells Promote Diabetic Corneal Epithelial Wound Healing Through TSG-6–Dependent Stem Cell Activation and Macrophage Switch.
Investigative Ophthalmology & Visual Science 5810:4064-4074 (2017) IF = 3.303 Year 2017
-
Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs.
BMC Musculoskeletal Disorders 18:423 (2017) IF = 1.739 Year 2017
-
Mechano-signal transduction in mesenchymal stem cells induces prosaposin secretion to drive the 1 proliferation of breast cancer cells.
Cancer Research 77:6179-6189 (2017) IF = 9.122 Year 2017
-
Intramembranous ossification and endochondral ossification are impaired differently between glucocorticoid-induced osteoporosis and estrogen deficiency-induced osteoporosis.
Sci Rep 8:3867 (2018) IF = 4.259 Year 2018
-
Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells.
Stem Cell Res Ther 1:31 (2019) IF = 4.963 Year 2019
-
Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow.
Exp Ther Med 16:5171-5177 (2019) IF = 1.41 Year 2019
-
Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3.
Experimental Cell Research PMID:31170401 (2019) IF = 3.309 Year 2019
-
C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction.
Cell Death & Disease 10:530 (2019) IF = 5.959 Year 2019
-
Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE PMID: 31782262 (2019) IF = 4.658 Year 2019
-
Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization.
Journal of Neuroinflammation DOI: 10.1186/s12974-020-1726-7 (2020) IF = 5.7 Year 2020