SD Rat Bone Marrow Mesenchymal Stem Cells With GFP&Luciferase

Introduction

Bone marrow mesenchymal stem cells (BMSCs) are a type of multipotent stem cell residing in the bone marrow stroma. Due to their strong proliferative capacity and immunomodulatory properties, BMSCs are widely applied in tissue engineering, cell therapy, and gene therapy.

As a research hotspot, SD rat bone marrow mesenchymal stem cells are commonly used in regenerative medicine and tissue engineering, particularly in studies involving bone, cardiovascular, and nervous system disorders.

OriCell™ SD Rat Bone Marrow Mesenchymal Stem Cells are isolated from the bone marrow of healthy SD rats. These cells exhibit robust proliferation and multipotent differentiation potential. Following adherent culture, they can be stably transduced with a lentiviral vector carrying GFP and Luciferase to achieve persistent co-expression of both genes. They serve as a versatile cell model for investigating proliferation, aging, immune regulation, differentiation, and transplantation.

When citing our products in academic publications, please use the following format: “OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences (Guangzhou) Inc.”

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
• Pass the viability examination. The viable rates is higher than 80%.
• The cell doubling time is less than 72 hours.
• Flow cytometry showed that CD29, CD44, CD90 are positive (>70%), while CD34, CD45, CD11b are negative (<5%).
• The cells can be induced to differentiate into osteoblasts, adipocytes, chondrocytes, etc.

Please refer to "COA" for details.

General Handing Principles

• Ensure that all equipment is kept clean and tidy.
• Please follow the instructions, strictly control the variables, and perform proper control experiments.
• Use suitable and reliable consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and their reuse is not recommended. The reagents used must be validated for reliability, suitable for cell growth, and exhibit minimal batch-to-batch variation.
• Bone marrow mesenchymal stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. OriCell™ SD Rat Bone Marrow Mesenchymal Stem Cells With GFP&Luciferase can be passaged for more than 5 times and still maintain all indicators qualified. But we always recommend using lower generation cells for scientific research.
• Usually the inoculation density of rat bone marrow mesenchymal stem cells is (2.5~4) ×10⁴ live cells/cm². The growth of these cells is strongly influenced by the donor's intrinsic characteristics as well as the subsequent culture system. We recommend adjusting the passage ratio according to the actual growth performance of each batch and passage.

Note: The cryopreservation solution of this product contains DMSO, which has potential risks. Please handle it carefully.