Wistar Rat Tendon Stem Cells

Introduction

Wistar rat tendon stem cells (TSCs) are a type of mesenchymal stem cell derived from Wistar rat tendon tissue, characterized by abilities such as self-renewal, multi-lineage differentiation, and colony formation. They are typically isolated from tendon tissue using a mixed enzymatic digestion method involving collagenase and neutral protease, and then cultured and expanded in a specialized culture medium.

Tendon stem cells have the potential to differentiate into multiple lineages, including tenocytes (tendon cells), chondrocytes, osteocytes, and adipocytes. They play a crucial role in tendon injury repair by promoting the regeneration of tendon tissue and the restoration of its function. The normal self-renewal and differentiation of tendon stem cells are essential for maintaining the structure and function of tendons. Abnormal differentiation of these cells may lead to tendon disorders, such as chondrification, ossification, and adipogenesis within tendon tissue, which can adversely affect tendon healing.

OriCell™ Wistar Rat Tendon Stem Cells, as a unique stem cell population, have become an important cellular resource in tendon tissue engineering and related disease treatment research due to their distinctive differentiation potential and key role in tendon repair.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >80%.
• Verified by cell cycle analysis, with a doubling time < 72 h.
• Verified by flow cytometry: Positive for CD29, CD44, and CD90 (> 70%); Negative for CD34, CD45, and CD11b (< 5%).
• Proven differentiation potential into osteoblasts, adipocytes, and chondrocytes.

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Prioritize low-passage cells. Generally, tendon stem cells have limited ability to proliferate in vitro and cannot maintain their differentiation potential for a long time. Leveraging our extensive cell culture expertise and optimized culture systems, OriCell™ Wistar Rat Tendon Stem Cells can be subcultured for more than 5 passages while maintaining their phenotypic integrity and meeting our rigorous quality control standards. However, we always recommend using low-passage cells for research applications.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ Wistar Rat Tendon Stem Cells is (2.5–4) × 10⁴ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.