Wistar Fetal Rat Hippocampal Neurons

Introduction

Neurons, a type of nerve cell, connect with each other to form neural networks. As the primary functional units of the nervous system, they serve as essential models for studying its function. Neurons are widely used in neurobiological research and the development of new treatments for Parkinson's disease and Alzheimer's disease.

OriCell™ Wistar Fetal Rat Hippocampal Neurons are derived from the cerebral cortex tissue of fetal rats at embryonic day 18.5 (E18.5), and are cryopreserved at the primary culture stage. They express specific neuronal proteins and can be used as a cell model for research on proliferation, aging, immunity, and other related fields.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >50%.
• Verified by immunofluorescence (IF): Positive for MAP2 and β-tubulin III (> 70%); Negative for GFAP (< 10%).

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
• Optimize seeding density and subculture. The recommended seeding density for OriCell™ Wistar Fetal Rat Hippocampal Neurons is (1–2) × 10⁵ viable cells/cm². Since cell growth is highly dependent on donor characteristics and culture conditions, we recommend adjusting the split ratios based on the actual performance of each specific lot and passage.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.