EN
IPEC-J2 Porcine Small Intestinal Epithelial Cell Line with RFP
Introduction
OriCell™ IPEC-J2 Porcine Small Intestinal Epithelial Cell Line is a non-transformed, continuously proliferating normal epithelial cell line derived from the jejunal epithelium of neonatal unsuckled piglets. It is capable of forming a structurally and functionally intact epithelial barrier, exhibiting excellent polarized differentiation and stable physiological properties. This cell line closely recapitulates the in vivo intestinal epithelial microenvironment and serves as a well-established in vitro model for studies of porcine and comparative intestinal biology.
Compared with tumor-derived cell lines (such as Caco-2), IPEC-J2 more accurately reflects the metabolic activity, barrier function, and host–pathogen response of normal intestinal epithelial cells, owing to its non-tumor origin. Therefore, it offers enhanced biological relevance for investigations into intestinal barrier function, nutrient absorption and transport, host–microbe interactions, and mechanisms of infection and inflammation. In addition, it is widely used in 3D cell culture systems and in vitro intestinal model development.
OriCell™ IPEC-J2 Porcine Small Intestinal Epithelial Cell Line with RFP is generated by transducing the parental cell line with a recombinant lentiviral vector encoding RFP, resulting in stable expression of the reporter gene. This cell line can be used for various in vitro and in vivo studies requiring cell tracking.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ IPEC-J2 Porcine Small Intestinal Epithelial Cell Line with RFP |
| Alternative Name | IPEC-J2 with RFP |
| Catalog Number | P1-0104 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Porcine Small Intestine |
| Cell Characteristics | Adherent Growth; Epithelial-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 24 ~ 48 h |
| Passage Number | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
