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C2C12 Mouse Myoblast Cell Line
Introduction
The C2C12 mouse myoblast cell line is a subclone derived from a mouse myoblast cell line originally established by D. Yaffe and O. Saxel. C2C12 cells can differentiate under low-serum starvation conditions, forming contractile myotubes and expressing characteristic muscle-specific proteins. Upon treatment with bone morphogenetic protein-2 (BMP-2), their differentiation pathway is redirected from myogenesis to osteogenesis.
OriCell™ C2C12 Mouse Myoblast Cell Line is widely used in skeletal muscle research, including studies on coagulation factor expression and the role of microRNAs in myogenic differentiation.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ C2C12 Mouse Myoblast Cell Line |
| Alternative Name | C2C12 |
| Catalog Number | M7-0101 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Skeletal Muscle; Strain: C3H |
| Cell Characteristics | Adherent Growth; Myoblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery testing, with a post-thaw viability of >90%.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
