Myotube Differentiation Medium For Muscle Cells

Introduction

OriCell™ Myotube Differentiation Medium For Muscle Cells, meticulously developed by our R&D team, is designed for the differentiation of muscle cells into myotubes. It provides a complete system, including an optimized basal medium, essential induction supplements and staining solution.

This product is effective for the myogenic induction of various muscle cells. Extensive cell culture data have demonstrated that it supports the stable and efficient differentiation of muscle cells (e.g., C2C12 cell line and primary skeletal muscle cells) into myotubes.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

*Note: RT = Room Temperature (15-30 °C).

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Pass the detection of osmotic pressure and pH.
• Pass the detection of product quality.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
• Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.

Product Stability and Storage Conditions

• All components must be stored away from light.
• The basal medium and staining solution have a shelf life of 1 year and should be stored at 4 °C and room temperature (RT) respectively. The supplement has a shelf life of 2 years and should be stored at -20 °C.
• Once prepared, the medium has a shelf life of 1 month when stored at 4 °C. The shelf life may be extended up to a maximum of 45 days, provided that culture conditions remain stable, the container is properly sealed, and repeated temperature fluctuations are avoided.
• Use all components before the expiration dates. Expired components may severely compromise culture performance.