N1-S1 Rat Liver Cancer Cell Line
Introduction
The N1-S1 rat hepatocellular carcinoma (HCC) cell line was first established by a Japanese research team in the 1970s. It is derived from Sprague-Dawley rats and was induced by feeding male rats 4-dimethylaminoazobenzene to develop hepatocellular carcinoma. Under in vitro culture conditions, the cells exhibit relatively stable morphology and biological characteristics, making them suitable for long-term experimental studies such as drug screening and gene function analysis.
OriCell™ N1-S1 Rat Liver Cancer Cell Line is commonly used to establish rat liver cancer models, facilitating studies on tumor growth and metastasis mechanisms. It is also widely applied in anticancer drug screening and efficacy evaluation, as well as in investigations of the regulatory mechanisms of liver cancer–related biomarkers.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ N1-S1 Rat Liver Cancer Cell Line |
| Abbreviation | N1-S1 |
| Alternative Name | N1-S1, ANA1 |
| Catalog Number | R1-0901 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Rat liver |
| Cell Characteristics | Suspension growth; circular clusters |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM+10% FBS |
| Doubling Time | 12~24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Tested for bacteria, fungi, mycoplasma, and endotoxins.
- Tested for cell viability and recovery.
- Confirmed by short tandem repeat (STR) analysis.
Please refer to "COA" for details.
General Handling Principles
- Strict sterile environment. Ensure the cleanliness of the entire laboratory, laminar flow hood, and incubator.
- Follow standardized operating procedures. Adhere to the instructions described in the product manual, strictly control variables, and conduct appropriate control experiments.
- Use appropriate and high-quality consumables and reagents. This product requires culture vessels suitable for suspension cell growth, and their reuse is not recommended. The reagents used must be validated for reliability, suitable for cell growth, and exhibit minimal batch-to-batch variation.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.