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MC3T3-E1 Subclone 14 Mouse Calvarial Preosteoblast Subclone 14 Cell Line with GFP
Introduction
The MC3T3-E1 Subclone 14 (mouse calvarial pre-osteoblast) is a clonal cell line derived from the phenotypically heterogeneous parental MC3T3-E1 cell population. This subclone was specifically selected from osteoblasts cultured in the presence of ascorbic acid for its high capacity for osteoblastic differentiation and mineralization.
OriCell™ MC3T3-E1 Subclone 14 Mouse Calvarial Preosteoblast Subclone 14 Cell Line with GFP is generated by infecting the parental cells with a recombinant lentiviral vector carrying the GFP gene, resulting in stable expression of green fluorescent protein. This cell line exhibits robust osteoblastic differentiation when cultured with ascorbic acid and 3–4 mM inorganic phosphate, and is capable of forming a well-mineralized extracellular matrix (ECM) after 10 days of culture. It enables convenient cell tracking and visualization both in vitro and in vivo.
In addition, MC3T3-E1 Subclone 14 cells express mRNA for osteoblast-specific markers, including bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone/parathyroid hormone-related protein receptor (PTH/PTHrP receptor). It has been reported that when implanted into immunodeficient mice, these cells can form bone-like tissue resembling woven bone. This cell line, particularly the ECM it produces, serves as an excellent in vitro model for studying osteoblast differentiation.
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Product Information
| Product Name | OriCell™ MC3T3-E1 Subclone 14 Mouse Calvarial Preosteoblast Subclone 14 Cell Line with GFP |
| Alternative Name | MC3T3-E1 Subclone 14 with GFP |
| Catalog Number | M7-0203 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Calvaria |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | MEM ·α＋10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If GFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
