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KLN 205 Mouse Lung Squamous Cell Carcinoma Cell Line with RFP
Introduction
KLN 205 Cell Line is derived from the lung tissue of a DBA/2 mouse with spontaneous lung squamous cell carcinoma. The cells exhibit a typical epithelial-like morphology, growing in adherent culture with tight intercellular junctions and a distinctive cobblestone appearance. As a highly tumorigenic and immortalized cell line, KLN 205 possesses a strong proliferative capacity. It can be maintained stably through continuous in vitro passages while retaining robust and reliable tumorigenic potential in vivo, making it an invaluable experimental tool in lung cancer research.
OriCell™ KLN 205 Mouse Lung Squamous Cell Carcinoma Cell Line with RFP is generated by infecting KLN 205 cells with a recombinant lentiviral vector carrying the RFP gene, resulting in stable expression of red fluorescent protein. It enables convenient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ KLN 205 Mouse Lung Squamous Cell Carcinoma Cell Line with RFP |
| Alternative Name | KLN 205 with RFP |
| Catalog Number | M0-0204 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Lung |
| Cell Characteristics | Adherent Growth; Epithelial-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 48 ~ 72 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
