EN
CT26.WT Mouse Colon Cancer Cell Line
Introduction
The CT26.WT Mouse Colon Cancer Cell Line is an undifferentiated colon carcinoma cell line induced by N-nitroso-N-methylurea (NNMU). The clonogenic cell line CT26.WT has been widely used as a model for evaluating immunotherapeutic strategies and studying host immune responses. CT26.WT cells were stably transduced with the retroviral vector LXSN carrying the lacZ gene encoding the prototypic tumor-associated antigen (TAA) β-galactosidase (β-gal), resulting in the highly tumorigenic subclone CT26.CL25.
OriCell™ CT26.WT Mouse Colon Cancer Cell Line is commonly used for the establishment of murine colorectal cancer models to investigate tumor growth, invasion, and metastasis. It serves as an important experimental model for basic colorectal cancer research, drug development, and the evaluation of therapeutic strategies, including studies on the immunomodulatory and antitumor effects of Shenshan Granules in CT26.WT tumor-bearing mice.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ CT26.WT Mouse Colon Cancer Cell Line |
| Alternative Name | CT26.WT |
| Catalog Number | M1-0101 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Colon |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | RPMI-1640 + 10% FBS |
| Doubling Time | 12 ~ 24 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
