EN
CT26.WT Mouse Colon Cancer Cell Line with RFP
Introduction
The CT26.WT Mouse Colon Cancer Cell Line is an undifferentiated colon carcinoma cell line induced by N-nitroso-N-methylurea (NNMU). The clonogenic cell line CT26.WT has been widely used as a model for evaluating immunotherapeutic strategies and studying host immune responses. CT26.WT cells were stably transduced with the retroviral vector LXSN carrying the lacZ gene encoding the prototypic tumor-associated antigen (TAA) β-galactosidase (β-gal), resulting in the highly tumorigenic subclone CT26.CL25.
OriCell™ CT26.WT Mouse Colon Cancer Cell Line with RFP is generated by infecting parental CT26.WT cells with a recombinant lentiviral vector carrying the RFP gene, resulting in stable expression of red fluorescent protein. This cell line is commonly used to establish murine colorectal cancer models for investigating tumor growth, invasion, and metastasis, and serves as an important experimental model for colorectal cancer research, drug development, and the evaluation of therapeutic strategies. In addition, it enables cell tracking in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ CT26.WT Mouse Colon Cancer Cell Line with RFP |
| Alternative Name | CT26.WT with RFP |
| Catalog Number | M1-0104 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Colon |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | RPMI-1640 + 10% FBS |
| Doubling Time | 48 ~ 72 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | Fluorescence Selection: If RFP fluorescence is weak, add puromycin (0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once fluorescence visibly increases. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
