BEND.3 Mouse Brain Microvascular Endothelial Cell Line

Introduction

OriCell™ BEND.3 Mouse Brain Microvascular Endothelial Cell Line is an immortalized endothelial cell line derived from the brain tissue of mice with endothelioma. It is one of the most commonly used endothelial cell lines for studies of cerebral vasculature and the blood–brain barrier (BBB). This cell line exhibits typical endothelial cell characteristics, grows as an adherent monolayer with a cobblestone-like morphology, and maintains stable proliferation over multiple passages. The cell line is robust and easy to culture, making it suitable for long-term experiments and large-scale drug screening.

This cell line demonstrates basic BBB barrier properties and responds sensitively to inflammatory cytokines (including TNF-α and IL-1β), enabling effective evaluation of barrier integrity and inflammatory regulation. It is widely applied in BBB permeability studies, drug and nanoparticle trans-barrier assessment, TEER measurement, and angiogenesis assays. Furthermore, BEND.3 cell line serves as a core component for constructing advanced in vitro models such as neurovascular units (NVU), 3D cell cultures, and microfluidic BBB chips.

All batches of the cell line undergo rigorous quality validation, including cell identity authentication, morphological evaluation, viability testing, and mycoplasma screening. This ensures high experimental reproducibility and data reliability. Due to its stability and robust modeling capabilities, BEND.3 cell line has become a widely used model for researchers in the fields of neuroscience and vascular biology.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing, with a post-thaw survival rate >90%.
• Verified by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.