B16-F10 Mouse Skin Melanoma Cell Line

Introduction

The B16-F10 mouse skin melanoma cell line was originally isolated in 1954 by researchers at The Jackson Laboratory from skin melanoma tissue of C57BL/6J mice. The cells exhibit a mixed spindle-shaped and epithelial-like morphology. The laboratory maintained B16 cells through continuous in vivo passage, and the B16-F10 subline was established from lung metastatic melanoma tissue obtained after the 10th consecutive passage of the parental B16-F0 cell line.

OriCell™ B16-F10 Mouse Skin Melanoma Cell Line is widely used for tumor model establishment and studies of tumor mechanisms, particularly in the fields of melanoma research and tumor immunology.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing.
• Verified by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.