A9 Mouse Subcutaneous Connective Tissue Cell Line

Introduction

The A9 Mouse Subcutaneous Connective Tissue Cell Line originates from mouse subcutaneous connective tissue cells derived from the L929 cell line, which was established in 1957 by George Yerganian's laboratory. This cell line is sensitive to HAT selective medium and lacks activities of adenosine phosphoribosyltransferase (APRT) and hypoxanthine-guanine phosphoribosyltransferase (HGPRT).

Due to HGPRT deficiency, A9 cells cannot survive in HAT medium. They are commonly used in fusion experiments with HGPRT-positive cells (such as tumor cells) to study the effects of chromosomal loss on cellular phenotype.

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Product Information

QC

• Tested for bacteria, fungi, mycoplasma, and endotoxins.
• Tested for cell recovery viability.
• Tested by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Strict sterile environment. Ensure the cleanliness of the entire laboratory, laminar flow hood, and incubator.
• Follow standardized operating procedures. Adhere to the instructions described in the product manual, strictly control variables, and conduct appropriate control experiments.
• Use appropriate and high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and their reuse is not recommended. The reagents used must be validated for reliability, suitable for cell growth, and exhibit minimal batch-to-batch variation.