A7r5 Rat Thoracic Aortic Smooth Muscle Cell Line
Introduction
The OriCell™ A7r5 Rat Thoracic Aortic Smooth Muscle Cell Line consists of fibroblast-like cells isolated from the thoracic aorta smooth muscle of embryonic rats. At confluence, these cells exhibit elevated myokinase and creatine phosphokinase (CPK) activity. Upon cessation of proliferation, A7r5 cells synthesize skeletal-type CPK.
A7r5 cells are commonly used in cancer research, including studies on the effects of compounds such as scutellarin on proliferation, migration, phenotypic modulation, and Notch1 signaling regulation in rat vascular smooth muscle cells.
When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences."
Product Information
| Name | OriCell™ A7r5 Rat Thoracic Aortic Smooth Muscle Cell Line |
| Alternative Name | A7r5 (A7R5) |
| Catalog Number | R2-0301 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Rat Thoracic Aortic Smooth Muscle |
| Cell Characteristics | Adherent Growth; Fibroblast-like |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 24~48 h |
| Biosafety Level | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | —— |
QC
- Tested for bacteria, fungi, mycoplasma, and endotoxins.
- Tested for cell recovery viability.
- Tested by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Strict sterile environment. Ensure the cleanliness of the entire laboratory, laminar flow hood, and incubator.
- Follow standardized operating procedures. Adhere to the instructions described in the product manual, strictly control variables, and conduct appropriate control experiments.
- Use appropriate and high-quality consumables and reagents. This product requires culture vessels suitable for adherent cell growth, and their reuse is not recommended. The reagents used must be validated for reliability, suitable for cell growth, and exhibit minimal batch-to-batch variation.