4T1 Mouse Breast Cancer Cell Line

Introduction

The 4T1 mouse breast cancer cell line is a 6-thioguanine–resistant cell line derived from the parental 410.4 tumor line and established without mutagenesis screening. The tumor growth and metastatic spread of 4T1 cells in BALB/c mice closely mimic those of human breast cancer. Tumors induced by 4T1 cells can serve as both postoperative and non-surgical models, as they spontaneously metastasize in both with similar kinetics.

OriCell™ 4T1 Mouse Breast Cancer Cell Line is widely used in cancer research, including studies on breast cancer development and metastasis mechanisms, screening and evaluation of anticancer drugs, investigation of drug resistance, and development and efficacy assessment of cancer vaccines. It provides an important tool and model for breast cancer–related research.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing.
• Verified by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for suspension growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.